摘要
以耐盐性强弱不同的野生大豆 (JWS耐盐性强 ,N2 32 32耐盐性弱 )为材料 ,采用砂培试验研究了NaCl胁迫下野生大豆叶片中Na+ 、Cl- 含量及活性氧代谢的变化。结果表明 ,盐胁迫下供试两种群野生大豆叶片Na+ 、Cl- 含量增加 ,N2 32 32比JWS更明显 ;耐盐性弱的N2 32 32叶片O- 2 产生速率和H2 O2 含量上升 ,膜脂过氧化产物MDA和电导率迅速上升 ,其叶片中Na+ 、Cl- 含量变化与O- 2 、H2 O2 、MDA和电导率增加呈极显著或显著正相关 ,而O- 2 产生速率和H2 O2 含量上升与MDA和电导率变化呈极显著正相关。表明在NaCl胁迫下 ,由于Na+ 、Cl- 在叶片中的积累而导致了活性氧的积累 ,活性氧诱发膜脂过氧化、破坏膜结构 ,导致膜结合酶H+ -ATPase和H+ -PPase活性降低 ,膜透性增加。耐盐种群JWS在盐胁迫下Na+ 、Cl- 积累量少、活性氧水平较低 ,对膜系统伤害少 ,膜结合酶活性高 ,因而能维持液泡膜正常的代谢功能 ,可能是其耐盐性强的原因之一。
The different salt tolerance of wild soybean (JWS salt-tolerant, N23232 salt-sensitive) were used to study the changes of Na +?Cl - contents and active oxygen metablism in the leaves under NaCl stress by the methods of pot experiment. The results showed that the Na +?Cl - contents increased in the leaves of the two population wild soybean under salt stress and the Na +?Cl - contents was greater in N23232 than that in JWS. For salt-sensitive wild soybean(N23232),the O -. 2 generation rate and H 2O 2 content increased, product of lipid peroxidation(MDA) and electrical conductivity increased in the leaves under 130 mmol/L NaCl stress. There were some significantly positive correlation among the Na +?Cl -?O -. 2?H 2O 2?MDA and electrical conductivity respectively, and also between O -. 2?H 2O 2 and MDA?electrical conductivity in the salt-sensitive wild soybean(N23232). It suggested that the content of Na +?Cl - increased lead to the accumulation of active oxygen and the oxidative damage. This oxidative damage expressed as the increase of MDA, the decrease of membrane-associated enzyme(H +-ATPase and H +-PPase) activities and the increase of electrical conductivity. However, the Na +?Cl - contents in the leaves of salt-tolerant wild soybean were maintained at a lower level, and no changes were detected in O -. 2?H 2O 2 and MDA contents with respect to the control. Therefore, the damage effects of active oxygen species were lower on the salt-tolerant wild soybean than that on the salt-sensitive ones, and the membrane-associated enzyme(H +-ATPase and H +-PPase) activities maintained at a higher level. The results indicated that the high level of H +-ATPase and H +-PPase activities in salt-tolerant wild soybean might be one of the factors of salt tolerance.
出处
《大豆科学》
CAS
CSCD
北大核心
2003年第2期83-87,共5页
Soybean Science
基金
8 6 3海洋生物技术项目 ( 2 0 0 1AA6 2 70 40 )
高等学校博士学科点专项基金 ( 19990 0 2 0 0 5 )资助。
关键词
野生大豆
耐盐性
活性氧代谢
膜结合酶
Wild soybean
Salt tolerance
Active oxygen metablism
Membrane-associated enzyme
