摘要
目的建立测定心脉康合剂中淫羊藿苷含量的反相高效液相色谱法,为质量控制提供依据。方法色谱柱为Diamonsil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.05%磷酸溶液(25∶75),流速为1.0 m L/min,检测波长为270 nm。结果淫羊藿苷进样量线性范围为0.062 6~1.879 8μg(r=1.000 0),精密度、稳定性及重复性的RSD均小于2.00%,平均回收率为99.35%,RSD为0.79%(n=9)。结论该方法简便、准确、重复性好,可用于该制剂质量控制。
Objective To establish a reverse phase- high performance liquid chromatography( RP- HPLC) method for the determination of icariin in Xinmaikang Mixture,so as to provide references for its quality control.Methods The HPLC analysis was carried out on the Diamonsil C18 column( 250 mm × 4.6 mm,5 μm).The mobile phase was consisted of acetonitrile- 0.05% phosphoric acid( 25 ∶75) with the flow rate of 1.0 m L / min.The detection wavelength was 270 nm.Results The linear range of icariin was 0.062 6 μg ~1.879 8 μg( r = 1.000 0),the RSDs of precision,stability and reproducibility tests were lower than 2.00%,with an average recovery rate of icariin 99.35%,RSD = 0.79%( n = 9).Conclusion This method is sensitive,simple and accurate with good repeatability and good linearity.It can be used for the quality contnet of icariin in Xinmaikang Mixture.
出处
《中国药业》
CAS
2015年第24期158-160,共3页
China Pharmaceuticals
关键词
心脉康合剂
淫羊藿苷
反相高效液相色谱法
Xinmaikang Mixture
icariin
reverse phase-high performance liquid chromatography
