摘要
Virus isolates, Y8, Y36 and Y38, were obtained from tobacco plants showing leaf curl symptoms in Honghe, Yunnan Province. In reactions with 14 monoclonal antibod-ies raised against Begomovirus particles, Y8, Y36 and Y38had similar antigenic reaction in TAS-ELISA as Tomato yel-low leaf curl China virus (TYLCCNV). The complete DNA-A nucleotide sequences of Y8, Y36 and Y38 were determinedand they contain 2727, 2730 and 2730 nucleotides, respec-tively. Each of the DNA-A sequences has a typical Bego-movirus genome organization encoding 6 ORFs with 2 ORFs [AV1(CP) and AV2] in virion-sense DNA and 4 ORFs (AC1to AC4) in complementary-sense DNA. Comparisons withtotal DNA-A, intergenic region and deduced amino acid se-quences of individual ORFs show that Y8, Y36 and Y38 are isolates of TYLCCNV. Satellite DNA molecules (DNAb) were found to be associated with Y8, Y36 and Y38, which consist of 1338, 1339 and 1338 nucleotides, respectively. Compari-sons show that these DNAb molecules share 98%—99% se-quence identities on nucleotide level and have a commonORF (designated C1) encoding 126 amino acids on the com-plementary strand.
Virus isolates, Y8, Y36 and Y38, were obtained from tobacco plants showing leaf curl symptoms in Honghe, Yunnan Province. In reactions with 14 monoclonal antibod-ies raised against Begomovirus particles, Y8, Y36 and Y38had similar antigenic reaction in TAS-ELISA as Tomato yel-low leaf curl China virus (TYLCCNV). The complete DNA-A nucleotide sequences of Y8, Y36 and Y38 were determinedand they contain 2727, 2730 and 2730 nucleotides, respec-tively. Each of the DNA-A sequences has a typical Bego-movirus genome organization encoding 6 ORFs with 2 ORFs [AV1(CP) and AV2] in virion-sense DNA and 4 ORFs (AC1to AC4) in complementary-sense DNA. Comparisons withtotal DNA-A, intergenic region and deduced amino acid se-quences of individual ORFs show that Y8, Y36 and Y38 are isolates of TYLCCNV. Satellite DNA molecules (DNAb) were found to be associated with Y8, Y36 and Y38, which consist of 1338, 1339 and 1338 nucleotides, respectively. Compari-sons show that these DNAb molecules share 98%—99% se-quence identities on nucleotide level and have a commonORF (designated C1) encoding 126 amino acids on the com-plementary strand.
基金
This work was supported by the National Outstanding Youth Foundation(Grant No.30125032)from the National Natural Science Foundation of China
the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of MOE.
关键词
番茄
中国
随体脱氧核糖核酸
烟草
病毒分离
Tomato yellow leaf curl China virus, DNAb, sequence,Begomovirus.
