摘要
目的探讨二氢杨梅素(DMY)对人乳牙牙髓干细胞(SHED)活性及成骨分化能力的影响。方法体外分离培养鉴定SHED,分别检测不同浓度的DMY对SHED的细胞活性、碱性磷酸酶(ALP)活性以及成骨相关基因Runt相关转录因子2(Runx2)和骨钙素(OCN) mRNA的表达的影响。结果 CCK-8检测结果显示,与对照组比较,0. 02、0. 1、0. 5、2、10及50μmol/L浓度的DMY对SHED的增殖能力差异无统计学意义;10、50μmol/L浓度的DMY ALP活性较对照组高(P <0. 05),0. 1、0. 5、2、10及50μmol/L DMY组的Runx2及0. 5、2、10、50μmol/L DMY组OCN成骨诱导前后相对倍增数均高于对照组(P <0. 05),并呈现一定的浓度依赖性。结论 0. 02、0. 1、0. 5、2、10及50μmol/L的(DMY)对SHED活性无明显影响,一定浓度的DMY可促进SHED成骨分化的能力。
Objective To observe the effect of Dihydromyricetin( DMY) on activity and osteogenic differentiation of stem cells from human exfoliated deciduous teeth( SHED) in vitro. Methods SHED were gained by Enzymatic digestion in vitro. The cellular viability,alkaline phosphotase activity,osteogenic differentiation related genes,such as Runt-related transcription factor 2( Runx2) and osteocalcin( OCN) were observed after different concentration of DMY act upon SHED. Results 0. 02,0. 1,0. 5,2,10 and 50 μmol/L concentration of DMY had no cytotoxicity to SHED. 10,50 μmol/L concentration of DMY increased the activity of alkaline phosphatase( P < 0. 05). 0. 1,0. 5,2,10 and 50 μmol/L concentration of DMY increased the mRNA expressions of Runx2,0. 5,2,10 and 50 μmol/L concentration of DMY increased the mRNA expressions of OCN which were in a concentration-dependent manner( P< 0. 05). Conclusion 0. 02,0. 1,0. 5,2,10,50 μmol/L concentration have no significant effect on the activity of SHED. A certain concentration of DMY can enhance the osteogenic differentiation of SHED.
作者
岳二丽
李夏宁
赵红宇
王一飞
刘飞
肖剑礴
Yue Erli;Li Xianing;Zhao Hongyu(Dept of Periodontics,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052;School of Stomatology,Zhengzhou University,Zhengzhou 450052;Dept of Stomatolagy,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052)
出处
《安徽医科大学学报》
CAS
北大核心
2019年第2期220-224,共5页
Acta Universitatis Medicinalis Anhui
基金
河南省高等学校重点科研项目计划(编号:17A320012)
河南省医学科技攻关计划项目(编号:201602015)
