摘要
目的探讨蛇葡萄素对实验性结肠炎模型大鼠血清和结肠IL-17A、IL-21、IL-23含量和结肠IL-17A、IL-21、IL-23 mRNA表达的影响。方法 SD大鼠随机分为正常对照组、模型对照组、美沙拉嗪对照组、蛇葡萄素低剂量组、蛇葡萄素中剂量组、蛇葡萄素高剂量组6组。以三硝基苯磺酸(TNBS)/乙醇复制实验性结肠炎大鼠模型,各组分别灌胃给药7 d,测定大鼠结肠湿重及长度、结肠组织中髓过氧化物酶(MPO)、血清和结肠中IL-17A、IL-21、IL-23含量和结肠IL-17A、IL-21、IL-23 mRNA表达。结果中、高剂量蛇葡萄素组和美沙拉秦组大鼠结肠湿重指数、结肠MPO显著低于模型对照组(<0.01);中、高剂量蛇葡萄素组和美沙拉秦组大鼠血清和结肠中IL-17A、IL-21、IL-23含量显著低于模型对照组(<0.01);中、高剂量蛇葡萄素组和美沙拉秦组大鼠结肠中IL-17A、IL-21、IL-23 mRNA表达显著低于模型对照组(<0.01);中、高剂量蛇葡萄素组和美沙拉秦组三组之间无显著性差异(> 0.05)。结论蛇葡萄素可下调血清和结肠中升高的IL-17A、IL-21、IL-23含量,下调结肠中升高的IL-17A、IL-21、IL-23 mRNA表达,调节IL-23/IL-17炎性轴功能失衡可能是其减轻结肠炎性损伤的机制之一。
Objective To investigate the therapeutic effects of ampelopsin(AMP)on experimental colitis in rats and its influence on interleukin-17A (IL-17A),interleukin-21(IL-21),interleukin-23(IL-23)content of serum and colon,and IL-17A, IL-21,IL-23 mRNA of colon.Methods Sprague-Dawley (SD)rats were randomly divided into normal control group,model control group,mesalazine group,and low,medium,high-dose ampelopsin (AMP)groups.Each group was administrated for 7 days.Wet weight and length of colon,myeloperoxidase (MPO)of colon,IL-17A,IL-21,IL-23 content of serum and colon, and IL-17A,IL-21,IL-23 mRNA of colon were measured.Results Wet colon weight index,MPO of colon,IL-17A,IL-21, IL-23 content of serum and colon,and IL-17A,IL-21,IL-23 mRNA of colon in medium,high-dose AMP and mesalazine group rats significantly lower than those of model group (P<0.01).And there were no statistically significant differences between the three group (P>0.05).Conclusion AMP attenuates TNBS/ethanol-induced colitis in rats and its efficacy is similar to mesalazine,one of the mechanisms might be related to adjustment of IL-23/IL-17 axis by decreasing IL-17A,IL-21 and IL-23 content of serum and colon,and IL-17A,IL-21,IL-23 mRNA of colon.
作者
张亚兵
张莹雯
艾望
柯浩亮
吴朝妍
夏玉坤
彭艳芳
叶太生
周珍
ZHANG Yabing;ZHANG Yingwen;AI Wang;KE Haoliang;WU Chaoyan;XIA Yukun;PENG Yanfang;YE Taisheng;ZHOU Zhen(Department of Integrated Chinese-Western Therapy,Zhongnan Hospital of Wuhan University,Wuhan 430071)
出处
《湖北中医药大学学报》
2018年第6期21-26,共6页
Journal of Hubei University of Chinese Medicine
基金
湖北省自然科学基金面上项目(项目编号:2017CFB772)
