摘要
AIM: To investigate the effect of liver nonparenchymal cell infusion combined with cyclosporin A (CsA) on rejection of heterostrain rat small bowel transplantation.METHODS: The liver nonparenchymal cell suspension was prepared by density gradient centrifugation method with Percoll centrifugal solution. Heterotopic small bowel transplantation was performed. Then the rats were divided into four groups. Group one: homogenic transplantation (F344/N→F344/N), group two: allotransplantation (F344/N →Wistar), group three: allotransplantation (F344/N→Wistar)+ CsA, with CsA 1O mg.kg-1.d-1 after transplantation, group four: allotransplantation + CsA (F344/N→Wistar) + liver nonparenchymal cell infusion + CsA (F344/N→Wistar), in which recipient Wistar rats had been injected with 2×108 F344/N liver nonparenchymal cells 20 days before transplantation, and treated with CsA after transplantation.Finally, the survival time after small bowel transplantation,gross and histopathological examination, and IL-2 levels in serum were observed.RESULTS: The survival time after small bowel transplantation was 7.14±0.33 d, 16.32±0.41 d and 31.41±0.74 d in group 2, 3, and 4, respectively. The survival time was significant longer (P＜0.01) in group 4. The gross and histopathological examination showed that the rejection degree in group 4 was lower than those in groups 2 and 3. Serum IL-2 level in group 4 was also lower than those in groups 2 and 3 (P＜0.01).CONCLUSION: Liver nonparenchymal cell infusion combined with CsA can prolong the survival time of rat small bowel transplantation, and the anti-rejection effect is good.
AIM:To investigate the effect of liver nonparenchymal cell infusion combined with cyclosporin A(CsA)on rejection of heterostrain rat small bowel transplantation. METHODS:The liver nonparenchymal cell suspension was prepared by density gradient centrifugation method with Percoll centrifugal solution.Heterotopic small bowel transplantation was performed.Then the rats were divided into four groups.Group one:homogenic transplantation (F344/N→F344/N),group two:allotransplantation(F344/N →Wistar),group three:allotransplantation(F344/N→Wistar) +CsA,with CsA 10 mg·kg^(-1)·d^(-1)after transplantation,group four:allotransplantation+CsA(F344/N→Wistar)+liver nonparenchymal cell infusion+CsA(F344/N→Wistar),in which recipient Wistar rats had been injected with 2×10~8 F344/N liver nonparenchymal cells 20 days before transplantation,and treated with CsA after transplantation. Finally,the survival time after small bowel transplantation, gross and histopathological examination,and IL-2 levels in serum were observed. RESULTS:The survival time alter small bowel transplantation was 7.14±0.33 d,16.32±0.41 d and 31.41±0.74 d in group 2,3,and 4,respectively.The survival time was significant longer(P<0.01)in group 4.The gross and histopathological examination showed that the rejection degree in group 4 was lower than those in groups 2 and 3.Serum IL-2 level in group 4 was also lower than those in groups 2 and 3(P<0.01).CONCLUSION: Liver nonparenchymal cell infusion combined with CsA can prolong the survival time of rat small bowel transplantation, and the anti-rejection effect is good.
基金
the National Natural Science Fundation of China,No.30070741
