摘要
利用全细胞膜片钳技术研究了铅对大鼠海马CA1区神经元瞬间外向钾电流 (IA)和延迟整流 (IK)的影响 .结果表明 ,铅可浓度依赖地抑制IA 和IK,半数抑制浓度分别为 37μmolL-1和 30 μmolL-1.此外还与电压呈依赖关系 .5 0 μmolL-1的Pb(Ac) 2 可非常显著地影响IA 和IK 的激活过程 ,还非常显著地影响IA 的失活过程 ,使激活和失活曲线均右移 .铅可抑制大鼠海马神经元的IA 和IK,并改变IA 和IK 的激活和失活过程 .这可能是铅损伤海马神经元的作用机制之一 .图 5参
The effect of lead on transient outward K + current ( I A) and delayed rectifier K + current ( I K) was investigated on freshly dissociated hippocampal CA1 neurons of rat using whole-cell patch-clamp techniques. The results showed lead reversibly inhibited the amplitudes of I A and I K in a dose-dependent and voltage-dependent. Halt-inhibit doses on I A and I K were 37 μmol L -1 and 30 μmol L -1,respectively. Lead could affect the activation process of I A and I K significantly. In addition,lead also affected the inactivation process of I A. Lead inhibited I A and I K in freshly dissociated hippocampal neurons of rats,which might damage the central neuronal system (CNS). Fig 5,Ref 16
出处
《应用与环境生物学报》
CAS
CSCD
2003年第6期619-622,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金资助项目 (30 0 70 640 )~~
关键词
海马CAl区神经元
瞬间外向钾电流
延迟整流钾电流
铅
全细胞膜片钳技术
hippocampal CA1 neurons
transient outward K+ current(IA)
delayed rectifier K+ current(IK)
lead
whole-cell patch clamp technique
