摘要
以载体 pCAMBIA2 30 1为骨架引入 pBI12 1中的GUS基因 ,构建了简便、实用的中间载体pCAMBIA2 30 1G .将其中一个GUS基因用目的片段盐藻烯醇酶基因替换 ,构建了可以在植物中高效表达的载体 pCAMBIA2 30 1G enolase,并将其转入根癌农杆菌EHA10 5中 ,采用叶盘转化法将盐藻烯醇酶基因转入模式植物烟草中 .Southern杂交结果显示 ,盐藻烯醇酶基因已整合到植物基因组中 ,在转基因烟草中的拷贝数为 2～ 4个 .图 6参
A simple and practical middle vector pCAMBIA2301G was constructed using pCAMBIA2301 which was inserted with GUS gene from the pBI121. Highly-expressed vector pCAMBIA2301G-enolase was constructed by replacing GUS gene with D.salina enolase gene. And pCAMBIA2301G-enolase was transformed to Agrobacterium tumefaciens strains EHA105 for transfection of tobacco. The Southern hybridization results showed there were 2～4 copies of Dunaliella salina enolase gene in the transgenic tobacco. Fig 6,Ref 10
出处
《应用与环境生物学报》
CAS
CSCD
2003年第6期603-606,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家转基因植物与产业化专项基金 (J0 0 A 0 0 8 0 9)
国家"863"项目资助 (2 0 0 1AA2 1 2 1 61 )~~
