摘要
目的:研究免疫耐受叙利亚仓鼠人胃癌异种移植模型对移植瘤的免疫耐受状态。方法:给新生24小时之内叙利亚仓鼠直接腹腔接种活的人瘤细胞建立模型,然后进行淋巴细胞转化试验、补体依赖性细胞毒试验,观察移植瘤内宿主淋巴细胞浸润情况。经给新生叙利亚仓鼠腹腔接种死的人瘤细胞诱导免疫耐受后,再于3周龄时皮下接种活的人瘤细胞观察致瘤情况,研究移植瘤的生长是否与肿瘤分泌的免疫抑制因子直接相关。结果:移植瘤细胞不能刺激荷瘤鼠淋巴细胞转化;荷瘤鼠血清不能引起对移植瘤细胞的补体依赖性细胞毒效应;移植瘤内未见宿主淋巴细胞浸润;新生叙利亚仓鼠腹腔接种死的人胃癌细胞诱导免疫耐受后,再于3周龄时接种活的人胃癌细胞可以致瘤。结论:本模型动物对移植瘤产生了特异性免疫耐受。
Objective: To study immune tolerance state to heterologous- transplanted tumor of human stomach cancer in Syria Hamsters. Methods: Syria hamsters within 24 hous after bom were intraperitoneally inoculated primary BCC-823 cells of human stomach cancer to establish the tumor model.Lymphocyte transformation test and complement dependent cytotoxicity test were performed. Lymphocytic infiltration from host in tumor transplanted was observed.Some neonatal Syria Hamsers were intraperitoneally inoculated dead primary cells of human stomach cancer to induce immune tolerance, and then re-inoculated subcutaneously live cells at three-week age. Tumor growth was observed and its relationship to immune inhibitory factor secreted from the tumor transplanted was studied.Results:Tumor cells from transplantation failed to induce the lymphocyte transformation of tumor-carrying Syrian hamsters and the serum from tumor-carrying Syrian hamsters couldn't cause complement dependent cytotoxicity to tumor cells of transplantation.The infiltration of of lymphocyte from host was not found in the tumor transplanted.The re-inoculation with live human stomach cancer cells in Syria hamsters at three-week age succeeded in inducing tumor after immune tolerance was induced by inoculatin dead cells. Conclusion: The Syria hamster after the inoculation of tumor cells has produced specific immune tolerance to transplanted tumor.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2004年第1期67-69,共3页
Chinese Journal of Immunology
基金
石家庄市科技局重点科研项目(99262065)
关键词
免疫耐受
叙利亚仓鼠
肿瘤
异种多植
模型
Immune tolerance
Syria hamster
Tumor
Heterologous transplantation
