摘要
目的:观察新生大鼠HIBD晚期不同bFGF治疗方案对caspase-3蛋白表达、DNA断裂的影响。方法:用免疫组化法观察caspase-3表达情况,用TUNEL观察神经细胞DNA断裂情况。结果:①bFGF1组caspase-3平均吸光度值(0.3571±0.0934)明显减弱,与对照1组(0.3706±0.0419)比较,两者间差异有显著性,P<0.05;②bFGF1组TUNEL阳性细胞数(4.20±1.30)亦明显少于对照1组(9.80±1.92),P<0.05;③bFGF2组、3组与其对照组比较则差异均无显著性,P>0.05。结论:bFGF可能为不断下调HI后caspase-3表达及随后的DNA断裂,因此应持续治疗方能发挥其神经保护作用。
Aim:To explore the effect of bFGF on caspase-3 brotein and DNA fragmentation in later time after neonatal rat hypoxic-ischemic brain damage.Methods:caspase-3 protein and DNA fragmentation were measured by using immunohistochemistry and TUNEL.Results:①caspase-3 average OD values were significantly lower in bFGF group 1 (0.357±0.093) compared with those in control group 1 (0.371±0.042) P < 0.05。 ②TUNEL positive cell counts were significantly lower in bFGF group 1 (4.20±1.30)compared with those in control group 1 (9.80±1.92), P < 0.05.③There were no differences among the other: two groups P > 0.05。Conclusion: It is suggested that bFGF might protect neuronal against apoptosis by down-regulation caspase-3 protein expression and followed DNA fragmentation,so a long and continue policy must be used.
出处
《中国临床神经科学》
2003年第4期354-356,共3页
Chinese Journal of Clinical Neurosciences
