摘要
目的 研究HBVC基因启动子 (BasicCorePromoter,BCP)基因变异与前S1(Pre -S1)抗原的关系以及BCP基因变异与HBVDNA含量的关系。方法 用PCR -微板核酸杂交ELISA技术检测 94例HBeAg阳性乙肝患者的BCP中nt 176 2A -T和nt 176 4G -A的基因突变 ,酶联免疫吸附分析 (ELISA)检测Pre -S1抗原 ,PCR荧光定量技术检测HBVDNA。结果 在 94例HBeAg阳性乙肝患者的血清中 ,Pre-S1抗原阳性有 6 5例 ,其中BCP阳性 5 1例 ,BCP变异率为 78.5 % ;Pre -S1抗原阴性有 2 9例 ,其中BCP阳性 2 3例 ,BCP变异率为 79.3%。BCP基因突变血清中HBVDNA拷贝数明显高于非BCP基因突变血清中HBV含量。结论 在e抗原阳性乙肝患者中 ,Pre -S1抗原的存在与BCP的变异无相关性 ,但BCP基因变异与HBVDNA拷贝数明显相关 ,可反映乙肝病情的严重程度及其愈后、转归。
Objective:To investigate the relationships between BCP mutation and both Pre-S1 antigen and HBV DNA concentration in HBV infected patients.Methods:ELISA was applied to detect Pre-S1 antigen, Fluorescence quantitative PCR was used to quantify the HBV DNA concentration, PCR-microplate nucleotide acid hybridization ELISA was used to detect the gene mutation of 1762(A-T) and 1764(G-A) in BCP in 94 HBeAg positive patients respectively.Results:Pre-S1 antigen was positive in 65 of 94 tested samples. BCP gene mutation had taken place in 51 of them and the positive rate was 78.5%. While 23 appeared to be BCP positive in 29 Pre-S1 antigen negative group and the mutation rate was 79.3%. HBV DNA concentrations in gene mutation group were significantly higher than those in BCP negative group.Conclusions:In HBeAg positive patients, the existence of Pre-S1 antigen was in no relation to BCP variation. But BCP variation was directly interrelated to the HBV DNA concentration;both of them can reflect the severity prognosis and of the disease.
出处
《中国现代医学杂志》
CAS
CSCD
2003年第23期15-17,共3页
China Journal of Modern Medicine
