摘要
目的 :探讨神经节苷脂 (GM1)对神经细胞的抗凋亡作用及其作用机制。方法 :复制大鼠中度脊髓损伤模型 ,随机分为对照组和GM1 治疗组 ,在蛛网膜下腔注射生理盐水或GM1,采用原位末端标记法、流式细胞仪AnnexinV/PI双标检测和测定Caspase3的活性来判断脊髓细胞凋亡情况。结果 :对照组和GM1 治疗组细胞凋亡均在治疗后3d达到高峰 ,但在治疗后1d～14d ,GM1 治疗组凋亡细胞显著低于对照组 (P<0 05～0 01) ,GM1 治疗组神经细胞的Caspase3活性明显低于对照组。结论 :GM1 可阻断神经细胞的凋亡 。
OBJECTIVE:To investigate the anti-apoptosis effect and the mechanism of GM 1 ganglioside on nerve cell afˉter the spinal cord injury.METHODS:The rats with compressed injury at the T8.9level of spinal cord were employed as the model.Then the rats were divided into two groups at random,one for the control and one for GMI treatment.The rats in each group were administrated normal saline solution(20μl)and GM 1 (30μg,20μl)intrathecally10minutes after injury,respectiveˉly.The apoptosis of nerve cells in the injured spinal cord were examined by TUNEL and flow cytometry with nerve cells labeled with Annexin V/PI;and Caspase3activity was measured by fluorometric immunosorbent enzyme assay.RESULTS:The apoptotic cells appeared in both groups at4h and reached their peak on3rd day after the injury.The proportion of apoptotic cells and the intracelluar Caspase3activity in the GM 1 treated group were significant lower than those in the control group(P< 0.05～0.01).CONCLUSION:GM 1 could inhibit the apoptosis of nerve cells and played a neuroprote ctive effects on the injured spinal cord.The mechanism may be resulted from reduction activation of intracellular Caspase-3in nerve cells of the injured spinal cord.
出处
《中国药房》
CAS
CSCD
2003年第12期717-719,共3页
China Pharmacy
