摘要
目的 评价选择性诱导型一氧化氮合酶 (iNOS)抑制剂氨基胍 (AG)在大鼠创伤性休克过程中的作用。方法 40只SD大鼠制作创伤性休克动物模型 ,然后随机分为休克组 ( 10只 )、AG组 (根据复苏时静脉注射AG含量为 2 ,8,60mg/kg则分为AGⅠ ,AGⅡ ,AGⅢ组 ,各 10只 ) ,观察休克前后血压及血浆NO浓度的动态变化及 2 4h大鼠存活率 ,并留取肺、肝、肾、小肠组织 ,观察病理改变。结果 大鼠创伤性休克后 ,血浆NO水平明显高于休克前 ;AG各组动物复苏后血浆NO的水平明显降低 ,各脏器的病理损害亦显著减轻 ,存活率明显提高 ,并且AGⅢ组效果最好。结论 NO在创伤性休克的病理发展过程中起着重要作用 ,应用AG有助于创伤性休克的改善 ,提高动物生存率。
Objective To evaluate the effects of aminoguanidine(AG), a selective inhibitor of inducible form of nitric oxide synthase(iNOS), on traumatic shock. Methods 40 Sprague-Dawley rats were used to establish models of traumatic shock. Animals were randomly divided into four groups: traumatic shock group(n=10) and AG group (group AGⅠ,AGⅡ and AGⅢ were infused with 2 mg/kg,8 mg/kg and 60 mg/kg of AG during resuscitation). Plasma levels of nitric oxide(NO) were determined before and after shock,immediately after resuscitation and 0.5 h, 2 h,4 h after resuscitation. The 24 h survivals were recorded. Lung, liver, kidney and intestine tissues were obtained 24 h after shock for microscopic examination.Results The plasma level of NO significantly increased after shock. The plasma level of NO significantly decreased and less tissue damages and higher survival tate were found in AG group. AGⅢ group had the best survival. Conclusion NO exerts an important role in development of pathological of traumatic shock. AG is beneficial for the treatment of traumatic shock and can improve the survival.
出处
《广东医学》
CAS
CSCD
2003年第12期1295-1297,共3页
Guangdong Medical Journal
基金
广东省自然科学基金资助项目 (编号 :0 0 1 0 4 8)
