摘要
【背景】随着中小城市经济的高速发展和人们生活水平的提高,生活污水排放量也日益增大,致使水源水质污染呈恶化趋势。【目的】了解湿地法处理生活污水对湿地地表水微生物群落多样性变化的影响。【方法】对人工湿地(仙桃)参照点(-2 000)、200、400、600、3 000、5 000 m水平方向6点地表水进行理化检测,再通过高通量测序对微生物群落结构进行解析,并探讨微生物群落结构变化与生活污水的污染效应。【结果】当湿地法处理的生活污水到达5 000 m时,化学需氧量、氨态氮、总氮和总磷均达《城镇污水处理厂污染物排放标准》一级标准,且理化指标之间均呈极显著正相关。样品在门水平上的优势物种分别为变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)、蓝藻门(Cyanobacteria)、疣微菌门(Verrucomicrobia)、厚壁菌门(Firmicutes)、酸杆菌门(Acidobacteria)和绿弯菌门(Chloroflexi)。变形菌门(Proteobacteria)可用来作为排放生活污水污染物的指示性微生物,放线菌门(Actinobacteria)、蓝藻门(Cyanobacteria)、疣微菌门(Verrucomicrobia)和绿弯菌门(Chloroflexi)可用来作为排放生活污水净化的指示性微生物。【结论】首次比较分析了湿地法处理仙桃生活污水地表水环境中微生物群落多样性变化的情况,可使人们从微生物层面更加明确地了解人工湿地运作机理及地表水环境的污染情况。
[Background] With the rapid development of economy in middle and small cities and the improvement of people’s living standard, Which the discharge of domestic sewage is also magnify,resulting in a worsening trend of water quality pollution. [Objective] To understand the effect of domestic sewage treatment on changes of microbial community diversity in wetland surface water.[Methods] Physical and chemical tests were conducted on 6 points of surface water in reference points(-2 000), 200, 400, 600, 3 000 and 5 000 m in constructed wetlands(Xiantao), Then the microbial community structure was analyzed by high-throughput sequencing, and the effects of microbial community structure change and domestic sewage pollution were also discussed. [Results] When the sewage treated by wetland method reached 5 000 m, Chemical oxygen demand(COD), NH4+N, Total nitrogen(TN) and Total phosphorus(TP) reached the first grade standard of pollutant discharge standard for urban sewage treatment plant, and there was a very significant positive correlation between the physical and chemical indexes. The dominant phyla in the studied surface water were Proteobacteria,Bacteroidetes, Actinobacteria, Cyanobacteria, Verrucomicrobia, Firmicutes, Acidobacteria and Chloroflexi. Proteobacteria can be used as an indicator microorganism for the discharge of pollutants from domestic sewage. Actinobacteria, Cyanobacteria, Verrucomicrobia and Chloroflexi can be used as an indicator microorganism for the purification of domestic sewage. [Conclusion] The changes of microbial community diversity in the surface water environment of Xiantao domestic sewage treatment were compared for the first time, which can make people understand the operation mechanism of constructed wetland and the pollution of surface water environment more clearly from the microbiological level.
作者
宋淑芬
杨帆
高绣纺
马立安
SONG Shu-Fen;YANG Fan;GAO Xiu-Fang;MA Li-An(College of Life Science,Yangtze University,Jingzhou,Hubei 434025,China;Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Education,Jingzhou,Hubei 434025,China;Wuhan TianYi HuiYuan Biotechnology Co.Ltd.,Wuhan,Hubei 430000,China;School of Earth Environment and Water Resources,Yangtze University,Wuhan,Hubei 430000,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2019年第3期512-521,共10页
Microbiology China
基金
国家自然科学基金(41371464)
长江大学留学基金~~
关键词
湿地法
生活污水处理
地表水
微生物
分洪道
Wetland method
Treatment of sanitary sewage
Surface water
Microbe
By-pass of flood
