摘要
本文应用鞭毛蛋白基因flaA和flaB的变性梯度凝胶电脉(denaturing gradient gel electrophoresis,DGGE)方法对食品中空肠弯曲菌和结肠弯曲菌进行检测和分型。采用RBB(rpeated bead beating)、CTAB和丙酮-氯仿抽提三种方法提取样品基因组后进行fla-DGGE检测,结果完全一致。10份生鲜鸡、鸭肉样品中有8份含有空肠弯曲菌或结肠弯曲菌,其中3份含有两种或两种以上的空肠弯曲菌或结肠弯曲菌或它们的不同型别。克隆测序后结果表明,有7份样品被同一种空肠弯曲菌所污染,其中3份样品还被同一种结肠弯曲菌所污染,1份样品被污染情况严重,检测到含有一种结肠弯曲菌和三种空肠弯曲菌。Fla-DGGE方法快速、准确、灵敏度高,可用于食品中空肠弯曲菌和结肠弯曲菌的快速检测和分型。
The aim of this study was to apply a denaturing gradient gel electrophoresis assay of the flagellin gene（fla-DGGE） for detection and genotyping of Campylobacter jejuni and Campylobacter coli in food samples without prior cμLtivation.RBB（rpeated bead beating）,CTAB and acetone-chloroform method were used to extracted DNA of all the samples,and then fla-DGGE was applied.The resμLts of the three methods were the same.10 samples,8 were with Campylobacter jejuni or Campylobacter coli,which 3 samples contaminated by two or more of the Campylobacter jejuni or Campylobacter coli,or their different types.After cloning and sequencing resμLts showed that 7 samples were contaminated with the same Campylobacter jejuni,including 3 samples also have the same type of Campylobacter coli,a serious pollution samples were detected to contain one Campylobacter coli and three types of Campylobacter jejuni.Fla-DGGE method is rapid,accurate,and sensitive and could be used in food in Campylobacter jejuni and Campylobacter coli in the rapid detection and typing.
出处
《现代食品科技》
EI
CAS
2010年第10期1148-1152,共5页
Modern Food Science and Technology
