摘要
食气梭菌是一类主要的化能自养微生物,可利用二氧化碳(CO_2)和一氧化碳(CO)合成多种化学品和燃料,具有良好的工业应用前景。天然的食气梭菌吸收、固定和转化一碳气体速率较慢,能量代谢效率低且高值产物种类少。近年来,随着组学、分子遗传学工具以及生化分析技术的快速发展,食气梭菌的生理代谢特点及其相关分子机制、代谢工程设计、改造和发酵工艺等方面都得到广泛而深入的研究。本文针对近年来食气梭菌的研究进展进行了梳理和总结,以期能为这类重要工业微生物的基础和应用研究,以及一碳气体的生物转化利用提供参考。
Gas-fermenting clostridia is a major class of chemoautotrophs that uses CO2 and CO tosynthesize a variety of chemicals and fuels, thereby exhibiting good industrial application prospects.Wild-type gas-fermenting Clostridium species are inefficient in gas uptake, fixation and conversion andenergy metabolism, and moreover, incompetent to produce high value products. In recent years, with therapid development of omics, molecular genetic tools and biochemical techniques in gas-fermentingclostridia of which the physiology and metabolism as well as the underlying molecular mechanisms,metabolic design and engineering, and fermentation technologies have been widely studied. Here, wereview the latest research progresses in gas-fermenting clostridia to provide information on these industrialmicroorganisms and bio-utilization of one-carbon gases.
作者
贾德臣
姜卫红
顾阳
JIA De-Chen;JIANG Wei-Hong;GU Yang(Key Laboratory of Synthetic Biology,Centre for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China;University of Chinese Academy of Sciences,Beijing 100039,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2019年第2期374-387,共14页
Microbiology China
基金
上海市科学技术委员会科研计划(17JC1404800)
国家自然科学基金(31630003)
中国科学院青年创新促进会优秀会员人才专项(2012213)
吐露港生物科技未来发展基金[2018-07(GY)]~~
关键词
食气梭菌
一碳气体
分子工具
遗传改造
发酵工艺
Gas-fermenting clostridia
One-carbon gases
Molecular tools
Genetic modification
Fermentation process
