摘要
目的 :分离并研究红栓菌胞外多糖的结构性质。方法 :红栓菌发酵液 ,滤去菌丝等固形物 ,浓缩发酵液至 1/ 4原体积 ,经乙醇沉淀、脱色、除蛋白、乙醇沉淀、干燥得多糖粗品PPS1 ;PPS1 经DEAE 纤维素DE 52以及SephadexG 150纯化得多糖纯品PPS2 ;采用凝胶过滤、醋酸纤维膜电泳、红外光谱、紫外光谱、核磁共振谱、气相色谱等方法测定多糖的性质。结论 :从红栓菌中获得的多糖PPS2 为单一组分 ,相对分子量为 2 92 0 0 ,由木糖、甘露糖。
OBJECTIVE:To isolate and study the polysaccharide from Pycnoporus cinnabarirus METHOD:The fermentative fluid was concentrated to 1/4 volume, precipitated by ethanol, decolorized and deproteinized by Sevag method, then a crude polysaccharide PPS 1, was obtained. After purification by ionexchange chromatography on DEAE cellulose DE 52 and molecular sieve chromatography on Seperdex G 150, the refined polysaccharide from PPS 1, named PPS 2, was obtained. PPS 2 was characterized by means of gel permeation chromatography, cellulose acetate membrane electrophoresis, IR, UV, 1 H NMR and gas chromatography. RESULT: PPS 2 was shown to be homogeneous, the relative molecular weight was estimated to be approximately 29 200,and the component monosaccharides were D xylose, D mannose, D glucose and D galactose.
出处
《中国天然药物》
SCIE
CAS
CSCD
2003年第4期243-245,共3页
关键词
红栓菌
胞外多糖
分离
纯化
性质
测定
Pycnoporus cinnabarirus
Exocellopolysaccharide
Purification
Characterization
