摘要
通过根癌农杆菌(Agrobacterium tume faciens)介导法将色氨酸单氧化酶(iaaM)基因和1-氨基环丙烷-1-羧酸(ACC)脱氨基酶基因转入矮牵牛,重金属抗性实验表明,表达iaaM基因的矮牵牛茎生长得到促进,生长速度快,但对钴毒害较为敏感;共同表达iaaM和ACC脱氨基酶基因的矮牵牛不但生长快,而且对钴毒害有较强的耐受性,在钴浓度为126μmol/L的生根培养基中能够正常生根。
The iaaM gene and ACC deaminase gene were introduced into Petunia hybrida by Agrobacte- rium tumefaciens,and the experiment of heavy metal resistance showed that the expression of iaaM gene can promote the growth of Petunia hybrida,transgenic plants grew more quickly than control plants,but became hypersensitive to cobalt poison.Transformed Petunia hybrida with both iaaM gene and ACC deaminase gene not only grew more quickly but also showed significant resistance to cobalt. The transgenic Petunia hybrida can root normally on MS rooting medium with cobalt 126μmol/L.
出处
《武汉生物工程学院学报》
2008年第3期128-132,共5页
Journal of Wuhan Bioengineering Institute
基金
湖北省青年杰出人才基金资助项目(2003AB016)
