摘要
以ACC（1-氨基环丙烷-1-羧酸）为唯一氮源，采用PAF、DF 和ADF培养基定向富集的方法筛选具有ACC脱氨酶耐盐活性的促生菌株，并确定其产酶条件。结果表明：筛选出53株活性菌株，其中jz1和jz5的活性较高，分别为0.2802U·mg^-1和0.2415U·mg^-1；其产酶条件的最适pH 值为7.5，最佳培养时间为24-42h，最适温度为25-30益，随着底物浓度的增加酶活性上升。筛选出的活性菌株对黄瓜种子均有不同程度的促生作用，其中jz1 和jz6 处理较为明显，处理后黄瓜根长103.19mm，下胚轴长52.89mm。
With 1-aminocyclopropane-1-carboxylic acid （ACC） as the only source of nitrogen, the methods of directional enrichment of PAF, DF and ADF medium were applied to screen the salt resistant growth-promoting strains of ACC deaminase, and determine the enzyme producing conditions. Fifty three strains were screened as active strains in which jzl and jz5 were the most activated,（0.2802±0.0033）U/mg and （0.2415±0.0058）, respectively. The screened active strains had different growth-promoting effects on cucumber seeds. Jzl and jz6 were more active than others, with the root length 103.19±0.17mm and 52.89＋0.06mm, respectively. The optimum pH and temperature of the enzyme producing conditions were 7.5 and 25-30℃ respectively, and the best incubation time was 24-42h. With the increasing of the substrate concentration, the enzyme activity would rise.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2013年第6期837-840,共4页
Journal of Shenyang Agricultural University
基金
辽宁(营口)沿海产业基地耐盐植物绿化工程项目(A20060921)
关键词
ACC脱氨酶
耐盐
促生菌株
产酶条件
ACC deaminase
salt-tolerance
growth-promoting strain
the enzyme producing conditions
