摘要
研究了木聚糖的聚合度和添加黄豆粉对里氏木霉合成木聚糖酶的影响,并以纯化木聚糖酶水解木聚糖。研究结果表明:木聚糖经酶水解后平均聚合度降低54%,戊聚糖含量为75.4%。采用低聚合度木聚糖为底物碳源和添加黄豆粉都可提高产酶效果。以12g/L低聚合度木聚糖添加2g/L黄豆粉,培养3d后木聚糖酶活力可达到113IU/mL,提高49.3%。通过对木聚糖酶进行纯化处理可以有效除去β 木糖苷酶。以体积分数10%的木聚糖酶水解35g/L木聚糖3h后,低聚木糖得率达到35.5%,而木糖得率仅为1.5%,低聚木糖与总糖的比值达到95.8%,随着酶解时间的延长,低聚木糖不会被降解。
In this paper,effects of the degree of polymerization(DP) of xylan and soybean powderadditive on the production of xylanase with Trichoderma reesei were studied.The result showed that the DP of xylan decreased 54% after enzymolysis with xylanase,and the content of lowpolymerized xylan was 75.4%.The production of xylanase with the lower DP xylan and soybean powderadditive was better than that of xylan,and the xylanases activity of Trichoderma reesei could be improved by 49.3% and reach 113 U/mL when cultured in 12g/L lower DP xylan residues and 2 g/L soybean powderadditive medium after 3 days.The exo1,4βDxyloisidase in xylanases could be deleted effectively with the separation technique.The yields of xylooligosaccharides and xylose were 35.5% and 1.5%,respectively,and the ratio between xylooligsaccharides and total sugar was 95.8% when 35 g/L xylan was hydrolysised with 10%(v/v) purified xylanase for 3 hours.Xylooligosaccharides would not be decomposed for longer reaction time.
出处
《林产化工通讯》
2003年第6期3-7,共5页
Journal of Chemical Industry of Forest Products
