摘要
用包埋前免疫电镜技术,研究以辣根过氧化物酶二次免疫后2、3、5、8和10天豚鼠腘淋巴结中抗体形成细胞的发育和分化。按照细胞的不同发育阶段和抗体阳性反应特征,可将它们分为四类细胞:(1)Ⅰ类细胞(淋巴细胞),核位于细胞中央,胞质很少,核周腔呈阳性反应,细胞质中有大量均匀分布的阳性颗粒;(2)Ⅱ类细胞(前浆细胞),核质比减低,有的核偏位,胞质中出现大量颗粒状短或棒状阳性反应物,其中有一些呈平行排列;(3)Ⅲ类细胞(前浆细胞),胞质中显示大量平行排列的阳性反应物;(4)Ⅳ类细胞(浆细胞),胞质中平行排列的阳性反应物连结成网状。根据细胞的形态以及胞质中阳性反应物从颗粒状、短棒状到平行排列的片层和连结成网状的变化,显示出从淋巴细胞经前浆细胞发育为成熟浆细胞,不断合成并累积抗体的动态过程。
The pre-embedding immunoelectron microscopic method was used to study the development of antibody-producing cells in the guinea pig popliteal lymph nodes of 2,3,5,8 and 10 days after a second challenge with horseradish peroxidase. The results indicated that the antibody activity was located in the perinuclear space, the endoplasmic reticulum and Golgi complex. According to the cellular developmental stages and the characteristics of distribution of the antibody activity, the antibody-producing cells (APC) were divided into four types: (1) Type Ⅰ cells (lymphocytes) exhibited many positive granules throughout the cytoplasm; (2) Type Ⅱ cells (proplasmacytes) contained many positive granules and positive short bars, some of them were parallel; (3) Type Ⅲ cells (proplasmacytes) contained numerous parallel positive lamellae in cytoplasm; (4) the parallel lamellae in cytoplasm of type Ⅳ cells (plasmacytes) were arranged into a network-endoplasmic reticulum. According to the kinetic change from granules, short bars to parallel iamellae and the network, the results indicated the developmental course of AFC from lymphocytes, proplasmacytcs to plasmacytes.
出处
《实验生物学报》
CSCD
1992年第4期295-303,共9页
Acta Biologiae Experimentalis Sinica
关键词
抗体形成细胞
发育
免疫电镜
Antibody-producing. Immunoelectren microseopy. Horseradish peroxidase. Popliteal lymph nodes of guinea pig.
