摘要
通过对伴或不伴心房颤动 (简称房颤 )的风湿性心脏病 (简称风心病 )二尖瓣病变患者左、右心房肌细胞缝隙连接蛋白 (Connexin ,Cx)Cx4 0、Cx4 3表达进行对比研究 ,探讨房颤对Cx表达的影响。选择 31例接受心脏瓣膜置换术的风心病患者为研究对象并分为三组 :实验Ⅰ组 11例 ,为阵发性房颤或房颤持续时间≤ 6个月者 ;实验Ⅱ组12例 ,房颤持续时间 >6个月 ;对照组 8例 ,为窦性心律。分别于手术中切取左、右心房肌标本。用免疫印迹法检测Cx4 0、Cx4 3的表达量。结果 :每例患者自身左、右心房肌比较 ,其Cx4 0及Cx4 3的表达量无显著差异 (P均 >0 .0 5 ) ;而每组中左、右心房肌Cx4 0、Cx4 3表达量均值也无显著性差异 ;其中实验Ⅰ、Ⅱ组与对照组比较 ,Cx4 0表达量明显降低 (左心房 :5 94 9.2± 832 .9,4 16 9.0± 95 5 .9Int×mm2 vs 86 74 .8± 10 0 8.3Int×mm2 ;P <0 .0 5和 <0 .0 1;右心房 :5 992 .1± 80 6 .5 ,4 12 0 .2± 95 4 .9Int×mm2 vs 86 17.4± 10 0 4 .7Int×mm2 ;P <0 .0 5和 <0 .0 1) ;Cx4 3的表达量在三组间无显著差异 (P >0 .0 5 )。结论 :Cx4 0表达下调可能参与了房颤的发生与维持。
The purpose of the study is to investigate the influence of atrial fibrillation(AF) to Cx40 and Cx43 in left and right atrial myocytes when the patients with rhematic heart disease suffered from AF or not. We investigated 31 patients with rheumatic heart disease undergoing cardiac surgery for correction to valvular defect. The patients with AF were divided into two groups according to the duration of AF: 11 of experimental Ⅰgroup were ≤6 months and 12 in experimental Ⅱgroup>6 months. 8 without AF were control group. Expression of Cx40 and Cx43 were detected by immunoblotting. Results: No obvious change was measured in the level of Cx43 and Cx40 between left and right atrium in each patients and every patients in each groups ( P >0.05). Comparing with the control group, the level of Cx40 was markedly decreased in experimental Ⅰand Ⅱgroup (Left atrium:5 949.2±832.9,4 169.0±955.9 Int×mm 2 vs 8 674.8±1 008.3 Int×mm 2, P <0.05 and <0.01,right atrium:5 992.1±806.5,4 120±954.9 Int×mm 2 vs 8 617.4±1 004.7 Int×mm 2, P <0.05 and <0.01). The level of Cx43 was similar in three groups. Conclusion: Down-regulation of level of Cx40 could be an important agent in the occurrence and maintenance of AF.
出处
《中国心脏起搏与心电生理杂志》
2003年第5期367-369,共3页
Chinese Journal of Cardiac Pacing and Electrophysiology
