摘要
目的 探讨三羟异黄酮 (genistein)对原癌基因 (HER -2 /neu)高表达乳腺癌血管形成的影响及其与血管内皮生长因子 (VEGF)的关系。方法 应用基因转染技术建立HER -2 /neu高表达的乳腺癌细胞 (MCF -7/HER -2 )。免疫缺陷裸鼠 (BALB/c)皮下接种乳腺癌 (MCF -7和MCF -7/HER -2 )细胞 ,其中接种MCF -7/HER -2细胞的裸鼠随机分为 3组 :对照组 ;genistein处理组 :HER -2 /neu抗体处理组。应用免疫组化法观察移植瘤内微血管密度(microvesseldensity ,MVD)和VEGF的表达变化。结果 MCF -7/HER -2移植瘤与MCF -7移植瘤相比血管密度较大 ,VEGF表达增强 ;MCF -7/HER -2移植瘤经genistein和HER -2 /neu抗体处理后 ,移植瘤的血管密度减少 ,VEGF表达降低。结论 HER -2 /neu高表达能促进乳腺癌的血管生成 ,genistein可能通过下调VEGF的表达从而抑制HER -2
Objective To explore the effect of genistein on angiogene sis in HER-2/neu-overexpressing breast cancer and the relationship with vascular endothelial growth factor.Methods HER-2/neu-overexpressing MCF-7 human breast cance r cells(MCF-7/HER-2) were generated by gene transfecting technique.MCF-7/HER-2 c ells and MCF-7 cells were injected into BALB/c nude mice.The nude mice with MCF- 7/HER-2 xenograft were randomly divided into three groups:control group,genistei n-treated group and HER-2/neu antibody-treated group.Microvessel density and VEG F expression exchang in xenograft tumors were analyzed with immunohistochemical staining.Results Microvessel density(MVD) and VEGF expression were significantly higher in MCF-7/HER-2 xenograft tumors than that in MCF-7 xenogra ft tumors.MCF-7/HER-2 xenograft tumors treated by genistein or HER-2/neu antibod y showed lower MVD and VEGF expression as compared with MCF-7/HER-2 xenograft tu mors.Conclusion The overexpression of HER-2/neu could promote angiogenesis in breast cancer,and genistein could inhibit angiogenesis in HER-2/ neu-overexpressing breast cancer through down-regulation expression of VEGF.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2003年第10期1168-1170,共3页
Chinese Journal of Public Health
基金
国家自然科学基金资助项目 (NO :30 0 0 0 1 36 )
