摘要
目的 :建立高效液相色谱测定母乳中奥硝唑浓度的方法。方法 :以C8 柱 (4 0mm×150mm ,5μm)为色谱柱 ,甲醇 -水(30∶70)为流动相。母乳样品用乙腈直接沉淀蛋白 ,无须经过有机溶剂提取直接进样分析。结果 :母乳中内源性物质不干扰奥硝唑的测定 ,奥硝唑在2 0～30 0μg/ml浓度范围内峰面积与浓度线性关系良好 (r=0 9997) ,最低检测限为0 49μg/ml。平均回收率为 (99 67±0 54) % ,高、中、低3种浓度的日内RSD≤2 5 % ,日间RSD≤2 1 %。结论 :本方法简便、快速、准确 ,重现性好 。
OBJECTIVE:To establish a HPLC method for determining the concentration of ornidazole in mother's milk METHODS:Stationary phase was Nova-Pak C8 column Mobile phase was methanol-water(30∶70) Samples were precipita_ted by acetonitrile without being extracted with organic solvent RESULTS:The endogenous substances in milk did not interfere with the determination of ornidazole The calibration curve was linear within the concentration range of 2 0～30 0μg/ml,r=0 9 997;the limit of detection was 0 49μg/ml;the average recovery was(99 67±0 54)%,and the within-day and between-day RSDs did not exceed 2 5% and 2 1%,respectively CONCLUSION:The method is simple,quick,accurate,sensitive,precise and usable for pharmacokinetic study of ornidazole in mother's milk
出处
《中国药房》
CAS
CSCD
2003年第11期675-676,共2页
China Pharmacy
