摘要
目的 :探讨补体C5a反义肽在C5a过敏毒素介导的肺血管内皮细胞与中性粒细胞粘附中的阻断作用。方法 :采用流式细胞术 ,检测C5a与其反义肽相互作用后再刺激人肺血管内皮细胞 (PVEC) ,对细胞表面粘附分子P选择素 (P Selec tin)表达的影响 ;同时通过测定粘附于血管内皮细胞表面的PMN中髓过氧化物酶 (MPO)活性 ,间接反映血管内皮细胞表面粘附的PMN数量的变化。结果 :发现反义肽R4可以剂量依赖关系抑制C5a介导的PVEC上粘附分子P Selectin的表达 ,当R4浓度为 5 0 0 0ng ml时 ,可使P Selectin的表达量下降 2 0 %左右 ,进一步增加R4的浓度 ,P Selectin表达量无显著性改变 ;另外 ,反义肽R4对PVEC与PMN的粘附也有明显抑制作用 ,表现为PVEC上粘附的PMN中MPO活性的降低 ,当R4的浓度增加到 5 0 0 0ng ml,MPO的活性可降低近 4 0 %左右。结论 :C5a反义肽对C5a过敏毒素的生物学活性具有一定的拮抗作用。
Objective:To study the blocking effects of anti-sense peptide of C5a on the adhesion between pulmonary vascular endothelial cell(PVEC) and neutrophil resulting from C5a anaphylatoxin.Methods:It was determined by Flowcytometry that the change of adhesion molecule expression on PVEC and the activity of MPO in PMN was determined after adhesion.Results:In response to C5a after interactions with several concentrations of anti-sense peptide R4, the expression of P-Selectin on PVEC decreased significantly and reached the minimum at the concentration of 5 000 ng/ml, and the activity of MPO in PMN reduced by 40% at the concentration of 5 000 ng/ml of anti-sense peptide R4.Conclusion:The results suggested that anti-sense peptide of C5a has significant blocking effects on C5a anaphylatoxin.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2003年第10期672-676,共5页
Chinese Journal of Immunology
基金
国家自然科学基金资助项目 ( 3 9970 3 3 0 )
关键词
反义肽
C5A
粘附分子
血管内皮细胞
髓过氧化物酶
Anti-sense peptide
C5a anaphylatoxin
Adhesion molecule
Vascular endothelial cells
MPO
