摘要
目的 :探讨心肌内注射碱性成纤维细胞生长因子基因 (b FGF基因 )治疗兔急性心肌梗死模型的效果。方法 碱裂解法大量制备质粒 ;采用开胸结扎兔冠状动脉左前降支 (L AD)法 ,建立兔急性前壁心肌梗死模型。模型制备成功后将动物分为治疗组 (n=19)和对照组 (n=18) ,并于心肌内分别注射 pc DNA3- b FGF10 0 μg和 pc DNA310 0 μg,饲养至 2 ,6 ,12周处死 ;免疫组化观察蛋白表达 ;行病理切片观察心肌梗死组织学变化和缺血心肌内血管新生的情况。结果 (1)术前术后描记的心电图证实兔急性心肌梗死模型制作成功 ;(2 )免疫组化观察注射 pc DNA 3- b FGF处心肌组织在 6周内有 b FGF蛋白的表达 ;(3)病理切片行图像分析计算血管密度发现 ,治疗组毛细血管密度和小动脉密度显著高于对照组。结论 心肌内注射 b FGF基因能促进缺血心肌内血管新生 。
Objective To study the effects of Naked DNA encoding bFGF for treatment acute myocardial infarction in rabbits model. Methods 42 rabbits underwent left thoracotomy followed by the ligation of left anterior descending coronary artery. After model reproducing, the rabbits were randomized to receive a directly intramyocardial injection of either pcDNA3 bFGF( n =19) or pcDNA3( n =18).2, 6 or 12 weeks later, immunhistologic analysis was performed to study expression of bFGF gene at protein level. histologic analysis was performed to evaluate angiogenesis induced by gene therapy. Results changes of electrocardiogram testify that the model of AMI is successful. immunhistologic analysis shows that bFGF gene can express in ischemic myocardial in 6 weeks. Histologic analysis shows:there was a significant increase in the density of capillaries and anterioles in gene therapy group. Conclusion Directly intramyocardial injection of bFGF gene can reduce the area of infart size and promote angiogenesis in ischemic myocardium.it is suggested that bFGF gene may have the possibility to become a new treatment strategy for coronary heart disease.
出处
《中国心血管杂志》
2003年第5期316-319,共4页
Chinese Journal of Cardiovascular Medicine
基金
云南省自然科学基金资助课题(编号 :2 0 0 0 C0 10 9M)
