摘要
目的研究碱性成纤维细胞生长因子(bFGF)在慢性乙型肝炎肝组织中的表达及其意义。方法用GAPDH作为参照,以RT-PCR对处于乙肝病毒(HBV)不同感染状态的体外培养肝细胞系以及人肝脏活检组织bFGFmRNA的表达水平进行半定量分析。结果在细胞水平和肝脏组织学两个层面上,HBV感染可影响bFGF的表达水平。正常肝细胞L02和持续性感染肝细胞HepG2.2.15细胞的bFGF/GAPDH分别为0.77±0.20、1.01±0.09;而在感染HBV患者肝组织和对照组其bFGF/GAPDH分别为0.78±0.14、0.50±0.14。持续性感染者bFGF表达量>未感染bFGF表达量(P<0.05)。结合临床病理显示:bFGF/GAPDH与纤维化组织学评分(HAI-F)呈正相关(r=0.3645,P<0.01)。结论bFGF在乙型肝炎持续性感染中表达上调,与疾病转归相关。
Objective To investigate the significance of bFGF expressed in the liver biopsies of chronic hepatiti B. Methods The RNA of hepatocytes in different infected patterns and the liver biopsies were extracted and semi-quantified by RT-PCR. Results HBV persistent infection could affect the expression of bFGF whether in the hepatocytes or in liver biopsies. The expression of bFGF in HepG2.2.15 cells（0.10±0. 09） was higher than that of in L02 cells（0.77±0. 20）. And at the same time the expression of bFGF in liver biopsies of chronic infection pattern （0.78±0. 14） was also higher than that of in uninfected pattern （0.50±0.14） （P〈0. 05）. Conclusion The expression of bFGF is up-regulated in the chronic hepatitis B and correlated to prognosis of HBV.
出处
《东南国防医药》
2007年第1期11-13,共3页
Military Medical Journal of Southeast China
