摘要
采用亲水电色谱对强极性吡啶类化合物进行了分离。以亲水性的强阳离子交换填料作固定相,乙腈 磷酸 三乙胺缓冲液作流动相,考察了流动相中有机改性剂含量、离子强度及流动相pH对吡啶类化合物保留行为的影响。结果表明,在亲水电色谱模式下,流动相中有机改性剂含量对吡啶类化合物保留有较大影响,随着流动相中乙腈含量的增加,吡啶类化合物的保留迅速增强;较高pH值有利于吡啶类化合物的峰形改善。实验证明,亲水电色谱是一种适用于极性化合物分离的新型微分离方法。
The separation of pyridine derivatives with hydrophilic interaction capillary electrochromatography (HICEC) on a hydrophilic, strongcationexchange stationary phase (Poly Sulfoethyl A) was investigated. The negative surface charge of this stationary phase provided a substantial cathodic electroosmotic flow (EOF). The effects of the acetonitrile content, the ionic strength and the pH value of mobile phase on the migration of pyridine derivatives were studied. The results indicated that the content of organic modifier has great influence on the retention behavior of pyridine derivatives and more symmetrical peaks can be obtained using the mobile phase with higher pH values. The satisfactory separation of pyridine derivatives has been achieved in this mode. It was shown that HICEC is a potential technique in the separation of polar compounds.
出处
《色谱》
CAS
CSCD
北大核心
2003年第5期516-519,共4页
Chinese Journal of Chromatography
基金
国家自然科学基金资助项目(项目号:20075032).
关键词
亲水作用
毛细管电色谱
吡啶类化合物
分离
capillary electrochromatography
hydrophilic interaction
pyridine derivatives
