摘要
以美国黄松成熟胚为外植体在GD+6-BA 0.5mg/L培养基进行离体培养诱导不定芽,附加20g/kg蔗糖、0.6g/kg琼脂,pH为5.8,研究不同成熟胚对组织培养的反应。试验结果表明,不同成熟胚的生理状态对组织培养具有重要的影响。
The mature embryos of Pinus ponderosa were used as explants to induce adventitious buds on GD + 6-BA 0. 5 mg/L medum. Influence of different mature embryos of P. ponderosa on tissue culture was studied. The results showed that influence of different mature embryos of P. ponderosa on tissue culture was important.
出处
《陕西林业科技》
2003年第3期5-7,共3页
Shaanxi Forest Science and Technology
基金
国家林业局"黄土高原林木培育实验室"资助项目"美国黄松组织培养及快繁技术研究"的内容
