摘要
目的 :探讨中药清肠栓治疗溃疡性结肠炎 (UC)的作用机理。方法 :用TNBS5 0 %乙醇溶液肛门注入制作大鼠UC模型 ,分组分别给予清肠栓、醋酸泼尼松等治疗 14d后处死 ,迅速剖取远端结肠 ,置液氮中备用。用RT -PCR、琼脂糖凝胶电泳及凝胶图象分析系统检测各组大鼠结肠粘膜IL - 1β、IL - 6mRNA的表达情况。结果 :清肠栓、醋酸泼泥松均可抑制模型大鼠结肠粘膜IL - 1β、IL - 6mRNA表达。结论 :IL - 1β、IL- 6是导致UC结肠粘膜损伤的重要炎症介质 ,清肠栓治疗UC的作用机理与抑制IL - 1β、IL -
Objective:To Study the mechanism of Chinese herbs qingchangshuan on ulcerative colitis(uc).Method:The UC models of rats were made by infusing the solution with TNBS 50% ethanol from the anus,the models were killed after being treated with Qingchangshuan 14 days and prednisone acetate respectively,and then their distal colons were extracted for preparation use in liquid nitrogen.and the IL-1β and IL-6mRNA expression of colon mucosa in each group were determined with RT-PCR,agarose gel electrophoresis and agarose gel image analysis system. Results: Qingchangshuan and prednisone acetate could inhibit both the IL-1β and IL-6mRNA expression of colon mucosa,prednisone acetate presented more significant function ( P <0 01),and the normal rats appeared no IL-1β and IL-6mRNA expression. Conclusion:IL-1β and IL-6mRNA expression are very important inflammation medium and the mechanism of Qingchangshang on UC is associated with its inhibition on IL-1β and IL-6mRNA expression.
出处
《中国中医药科技》
CAS
2003年第5期263-265,共3页
Chinese Journal of Traditional Medical Science and Technology
基金
上海市卫生局中医特色治疗专科资助项目
