摘要
目的 观察低水平铅暴露对生长发育期大鼠不同脑区一氧化氮合酶 (NOS)活力的影响。方法 SD大鼠经饮含 0 .0 2 5 %、0 .0 5 0 %、0 .0 75 %醋酸铅水方法染毒 ,建立生长发育期低水平铅中毒的大鼠模型 ,应用荧光测定法测定大鼠海马、小脑、大脑皮层、脑干的NOS活力。结果 出生 2 1d ,3组铅暴露组海马、小脑NOS活力 [海马 :(1.5 3± 0 .2 0 )、(1.6 6± 0 .2 3)、(1.88± 0 .32 )U mgpro ,小脑 :(0 .87± 0 .2 4 )、(0 .85± 0 .0 9)、(0 .91± 0 .18)U mgpro]明显低于对照组 [海马 :(2 .36± 0 .18)、小脑 (1.4 1± 0 .18)U mgpro](海马F =14 .4 15 ;小脑F =7.933,均P <0 .0 1) ;出生 7、14、2 1d ,0 .0 75 %醋酸铅组大鼠大脑皮层NOS活力 [7d :(1.2 9± 0 .14 )、14d :(1.0 3± 0 .15 )、2 1d :(0 .6 9± 0 .10 )U mgpro]明显低于对照组 [7d :(2 .5 4± 0 .31)、14d :(1.6 4± 0 .2 2 )、2 1d :(1.2 4± 0 .14 )U mgpro],0 .0 2 5 %组 [7d :(2 .4 2±0 .19)、14d :(1.5 9± 0 .17)、2 1d :(1.2 7± 0 .12 )U mgpro],0 .0 5 0 %组 [7d :(2 .5 6± 0 .5 3)、14d :(1.77±0 .19)、2 1d :(1.2 4± 0 .10 )U mgpro](F =4 2 .6 30 ,P <0 .0 5 ) ,对照组、0 .0 2 5 %组、0 .0 5 0 %组之间两两比较 。
Objective To observe the influence of low-level lead exposure on the activity of nitric oxide synthase(NOS) in different brain regions of rat. Methods By establishing a series of rat models exposed to different low levels of lead(drinking water containing 0.025%,0.050%,0.075% of lead acetate) during developing period, NOS activities in hippocampus,cerebellum,cerebral cortex and brain stem were studied. Results On the 21st day after birth,NOS activities in hippocampus of three levels of lead exposed groups[(1.53±0.20),(1.66±0.23),(1.88±0.32)U/mg pro respectively],and in cerebellum[(0.87±0.24),(0.85±0.09),(0.91±0.18) U/mg pro respectively] were significantly lower than those of control group[(2.36±0.18),(1.41±0.18)U/mg pro,respectively, P <0.01].NOS activities in cerebral cortex of 0.075% group [at 7,14,21 d of age[(1.29± 0.14) ,(1.03±0.15),(0.69±0.10)U/mg pro] were significantly lower than those in control group[(2.54± 0.31) ,(1.64±0.22),(1.24±0.14)U/mg pro respectively],and 0.025% group[(2.42±0.19),(1.59± 0.17), (1.27±0.12)U/mg pro respectively],and 0.050% group[(2.56± 0.53), (1.77±0.19),(1.24± 0.10) U/mg pro respectively, P < 0.05] .There were no significant differences among control,0.025%,and 0.050% groups( P >0.05).Lead exposure had no influence on NOS activity in brain stem at the same age( P > 0.05). Conclusion NOS activities in hippocampus,cerebellum and cerebral cortex were inhibited by low level lead exposure and the degree of the effect was related to Pb exposure time and(or) level of Pb exposed.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2003年第4期263-265,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
