摘要
目的 :采用大鼠海马神经元作为研究对象 ,研究咪唑安定对神经元钠离子通道的影响 ,以探讨其临床遗忘作用的可能机制。方法 :在急性分离的大鼠海马锥体神经元上 ,利用全细胞膜片钳技术 ,记录钠离子通道的电流 ,研究咪唑安定对钠离子通道电流幅度和激活特性的影响。结果 :咪唑安定对海马钠离子通道电流具有可逆性抑制作用 ,0 .1和1 0 μmol·L- 1 的咪唑安定对钠离子通道分别抑制了(7.95± 7.46) %和 (2 5 .3 7± 8.3 1 ) % (P <0 .0 1 )。1 0 μmol·L- 1 的咪唑安定还可使钠离子通道的激活曲线右移。结论 :咪唑安定对海马神经元上的钠离子电流有较弱程度的抑制作用 。
AIM: To study the effects of midazolam on sodium channels of hippocampal pyramidal neurons, and to investigate mechanisms whereby midazolam mediates amnesia. METHODS: Sodium current was recorded by whole cell patch clamp technique in the acutely isolated neurons. Size of currents and steady activation curve were measured. RESULTS: Sodium channels were inhibited by midazolam in a reversibly manner. The sodium peak current was reduced by ( 7.95 ± 7.46 )% and ( 25.37 ± 8.31 )% (P< 0.01 ) at 0.1 and 10 μmol·L -1 of midazolam, respectively. The steady activation curve shifted right significantly caused by 10 μmol·L -1 midazolam. CONCLUSION: Midazolam inhibits sodium current slightly, suggesting that effects of midazolam on sodium channels are not the main mechanism in amnesia.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2003年第5期515-517,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
药效学
咪唑安定
海马
锥体神经元
钠离子通道
膜片钳
pharmacodynamics
midazolam
hippocampus
pyramidal neurons
sodium channels
patch clamp
