摘要
应用膜片钳全细胞法，研究豚鼠单个心室肌细胞的钠离子通道电流（ＩＮａ）及其动力学特征。研究显示：ＩＮａ具有电压和时间依赖性，ＩＮａ在－５０ｍＶ激活，在＋４０ｍＶ反转。钠通道动力学包括稳态灭活曲线、使用依赖性阻滞及钠通道灭活后的恢复过程，对其特点的初步讨论表明，Ⅰ类抗心律失常药物与受体亲和力、失活态钠通道受体解离的时间常数，以及其使灭活曲线向左移的电位不同，可以作为Ⅰ类药物亚类的分类基础。
Sodium channel current (INa) and kinetics were investigated using patch clamp whole-cell recording in isolated single ventricular cells from adult guinea pig hearts.The study showed that INa was voltaged- and time-dependent,which was activated at-50mV and reversed at +40mV.The kinetics of sodium channel,including steady-state inactivation,use-dependent block and the time course of recovery from inactivated sodium channel,were discussed and analysed.The study also suggested that the differences of class Ⅰ antiarrhythmic drugs in receptor affinity,time constants for the dissociation from the inactivated receptor as well as potential shift of the inactivated curve coluld be used to subdivide these drugs.
出处
《南通医学院学报》
1998年第2期154-157,共4页
ACTA Academiae Medicinae Nantong
关键词
膜片钳
心肌细胞
钠通道
动力学
抗心律失常药
whole-cell patch clamp recording ventricular cell sodum channel kinetics
