摘要
目的观察温阳振衰颗粒含药血清对H9C2心肌细胞损伤模型miR-155/P38MAPK表达的影响。方法 H9C2心肌细胞分为正常对照组,正常+含药血清组,转染组,转染+含药血清组,阿霉素(ADR)组,ADR+含药血清组,ADR+转染组,ADR+转染+含药血清组。ADR组的培养基中加入2.67μmol/L的ADR,含药血清组培养基中加入10%的温阳振衰颗粒含药血清,共培养44h。检测实验末各组细胞miR-155和P38MAPK的表达水平。结果与正常对照组相比,ADR组可显著下调miR-155的表达,同时上调P-P38MAPK的表达,差异具有统计学意义(P<0.05)。转染组和含药血清组可明显上调miR-155的表达,同时下调P-P38MAPK的表达,差异具有统计学意义(P<0.05)。结论温阳振衰颗粒能上调miR-155的表达从而抑制P38MAPK蛋白的过度磷酸化,这可能是其治疗慢性心衰的重要机制之一。
Objective To elucidate the effects of Wenyangzhenshuai granule containing serum on miR-155/P38 MAPK regulates H9 C2 myocardial cell injury model.Methods H9 C2 myocardial cells were divided into normal control group,normal+drug serum group,transfection group,transfection+drug serum group,ADR group,ADR+drug serum group,ADR+transfection group,ADR+transfection+drug serum drug.Adding 2.67μmol/L ADR in H9 C2 myocardial cells and 10%drug serum,coculture for 44 hours.miR-155 and P38 MAPK expressions in these groups were detected by Western blotting and RT-PCR.Results Compared with the normal control group,the expression of miR-155 was significantly down regulated in the ADR group,and the expression of P-P38 MAPK was up-regulated,the difference was statistically significant(P<0.05).Transfection group and drug serum group could significantly increase the expression of miR-155,and down regulate the expression of P-P38 MAPK,the difference was statistically significant(P<0.05).Conclusion Wenyangzhenshuai granule can up regulate the expression of miR-155 and inhibit the excessive phosphorylation of P38 MAPK protein,which may be one of the important mechanisms in the treatment of chronic heart failure.
作者
蔡虎志
陈新宇
徐则林
吴治谚
CAI Hu-zhi;CHEN Xin-yu;XU Ze-lin;WU Zhi-yan(First Affiliated Hospital of Hunan University of Traditiona Chinese Medicine,Changsha 410007,China;Jiangmen Wuyi Traditional Chinese Medicine Hospital,Jiangmen,Guangdong,529000,China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2019年第2期257-260,共4页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(81704061
81173213)
湖南省中医药管理局资助项目(201651)
