摘要
目的 :观察石杉碱甲透皮控释贴片 (Hup ATDDS)督脉穴位给药对东莨菪碱 (Scop)致小鼠学习记忆障碍的影响 ,比较穴位给药与一般部位给药的作用差异 ,探讨督脉穴位给药机理 ,为穴位透皮控释给药新途径提供初步实验依据。方法 :采用跳台、“Y”迷宫等行为药理学实验方法 ,观察Hup ATDDS穴位给药与一般部位给药对Scop小鼠记忆障碍的改善作用 ,并以小鼠脑胆碱酯酶活性等指标初步探讨穴位给药的作用机理。结果 :跳台试验和电迷宫试验表明 ,Hup ATDDS督脉百会穴用药后 3～ 6h间有明显的改善学习记忆作用 :13.4 μg/ 0 .2 5cm2 组用药后 4h为佳 ,5～ 6h时作用减弱 ;2 6 .7μg/ 0 .2 5cm2 组自药后 3h起作用明显 ,药后 4h作用最为显著 ,维持至 6h仍有良好作用 ;Hup ATDDS 2 6 .7μg/0 .2 5cm2 经百会、哑门、大椎、至阳和命门五个穴位给药均有明显的改善学习记忆作用 ,其中以百会穴组的作用最为显著 ;Hup ATDDS经百会穴或一般部位 (选臀位 )给药均有改善学习记忆作用 ,但以百会穴给药较臀位用药作用更为显著 ,且Hup ATDDS2 6 .7μg/ 0 .2 5cm2 组对小鼠脑胆碱酯酶活性有明显抑制作用。结论 :Hup ATDDS督脉穴位给药的改善记忆作用优于一般部位给药 ;其改善学习记忆作用可能是由于抑制脑CHE活性 。
AIM: To observe the improvement function of huperzine A transdermal drug delivery system (Hup A TDDS) administration at Governor Vessel(GV) acu points on memory disorders induced by scopolamine in mice and to compare the effect of GV acu points administration with general place administration. METHODS: Step down test and 'Y' maze test were used to observe the influence of Hup A TDDS administration at GV acu points on memory disorders induced by scopolamine. Some biochemical indices were also determined. RESULTS: (1) The marked effects were found among 3- 6 h after Hup A TDDS administration at GV baihui acu point. The best effects showed 4 h after administration, and it weakened a little from 5 h to 6 h in 3.4 μg/ 0.25 cm 2 group. At 26.7 μg/ 0.25 cm 2 group, the drug action maintained an effective level from 3 h to 6 h, and reached the maximum at 4 h. (2) Among 5 acu points of GV to administration at Hup A TDDS 26.7 μg/ 0.25 cm 2, the obvious effects on memory disorders existed, and baihui (GV20) was the most effective acu point. (3) The administration of Hup A TDDS ( 26.7 μg/ 0.25 cm 2 and 13.4 μg/ 0.25 cm 2) through both acu point and general place (buttocks), approved memory improvement function by step down test and 'Y' maze test, and the action in baihui acu point group showed more significant. The depressed trend of cholinesterase activity in brain was proved in all of both GV acu point and general place groups and baihui acr point group exposed signal difference when compared with mokcl group. CONCLUSION: Hup A TDDS administration at GV acu point shows a better improvement of memory disorders in comparison with general place. The action mechanism may be due to the depressed cholinesterase activity and the improved cholinergic system function.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2002年第6期489-492,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家中医药管理局青年基金 (GQ2 0 0 0 14 )
浙江省自然科学基金 (JJ2 0 0 1 3 0 14 46)项目
关键词
石杉碱甲透皮控释制剂(贴片)
督脉
穴位
一般部位
记忆障碍
胆碱酯酶
huperzine A transdermal drug delivery system(Hup A TDDS)
Governor Vessel
acu point
general place
memory disorders
step down test
'Y' maze test
cholinesterase
