摘要
目的建立高效液相色谱法(HPLC)同时测定石龙清血颗粒中没食子酸、原儿茶酸、马钱苷、芦丁的含量。方法采用高效液相色谱法,Kromasil C18(250 mm×460 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸水(B)为流动相进行梯度洗脱,流速为1.0 mL·min-1,柱温为30℃,检测波长为240 nm。结果石龙清血颗粒中没食子酸、原儿茶酸、马钱苷、芦丁的线性范围分别为33.4~100.2、15.0~44.8、82.0~244.9、450.0~1343.7 mg·L-1,相关系数r均大于等于0.999 0,加样回收率均符合要求,含量分别为0.6577、0.2662、1.195和6.652 mg·g-1,含量RSD分别为0.9%、1.0%、1.4%和1.2%。阴性对照无干扰,各峰分离度良好。结论所建立的方法适用于石龙清血颗粒的质量控制。
Objective To establish a high performance liquid chromatography(HPLC)method to determinate the content in Shilongqingxue Granules(gallic acid,protocatechuic acid,loganin and rutin).Methods The column was Kromasil C18(250 mm×4.6 mm,5μm):acetonitrile(A)-0.1%phosphoric acid(B)was used as mobile phase in gradient elution:the flow rate was 1.0 mL·min-1:column temperature was 30°C:the detection wavelength was 240 nm.Results The linear ranges of gallic acid,protocatechuic acid,loganin and rutin in Shilongqingxue Granule were 33.4-100.2、15.0-44.8、82.0-244.9、450.0-1 343.7 mg·L-1,respectively.The correlation coefficient r was greater than or equal to 0.999 0.The recovery rates of the samples were all between 95%and 105%:the contents were 0.6576,0.266 2,1.195,and 6.652 mg·g-1,respectively,and the RSD were 0.9%、1.0%、1.4%and 1.2%,respectively.The negative control had no interference and the peaks were well separated.Conclusion This method is applicable to the quality control of Shilongqingxue granules.
作者
庄凤志
赵悦新
李艳华
席鹏飞
赵旻
赵春杰
ZHUANG Fengzhi;ZHAO Yuexin;LI Yanhua;XI Pgfei;ZHAO Min;ZHAO Chunjie(Sunflower Pharmaceutical Group(Jilin)Linjiang Co.,Ltd.,Linjiang134613,China;School ofPharmacy,Shenyang Pharmaceutical University,Shenyang110016,China)
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2019年第6期487-491,共5页
Journal of Shenyang Pharmaceutical University
关键词
高效液相色谱
石龙清血颗粒
没食子酸
原儿茶酸
马钱苷
芦丁
含量测定
high performance liquid chromatography
Shilongqingxue granules
gallic acid
protocatechuic acid
loganin
rutin
content determination
