摘要
利用96孔板,通过菌株与毒素共培养的方法,从217株芽孢菌中,经初筛和复筛得到两株具有很好降解效果的菌株,即T-246和T-420,它们分别能够在6 h和9 h内将15μg/m L的玉米赤霉烯酮完全降解。通过16S r DNA序列分析鉴定,T-246和T-420分别为同温层芽孢杆菌(Bacillus stratosphericus)和短小芽孢杆菌(Bacillus pumilus)。对其降解机理的初步研究表明,这两株菌对玉米赤霉烯酮都具有一定程度的吸附作用,但主要作用源于胞外酶的酶促降解。
Two bacillus T- 246 and T- 420,which possess very good degradation effect,have been screened from 217 strains of bacillus,which were cultured in 96- well micro plate with strain and viral co- culture,by primary screen and rescreen. It was confirmed that 15 μg / m L zearalenones( ZEN)could be degraded by these strains within 6 h and 9 h,respectively. These two strains were identified as Bacillus stratosphericus and Bacillus pumilus based on 16 S r DNA sequence analysis. In addition,the preliminary study on their degradation mechanism showed that they were able to bind ZEN,but the main effect of scavenging ZEN was due to the degradation activity of extracellular enzyme.
出处
《粮油食品科技》
2015年第3期90-94,共5页
Science and Technology of Cereals,Oils and Foods
基金
国家自然科学基金(31302007)
"十二五"农村领域国家科技计划课题(2011BAD26B002-5)
