摘要
阴沟肠杆菌w10j15经过纯培养、超声波破碎和高速离心，提取到氯氰菊酯降解酶。在实验条件下测定了降解酶对氯氰菊酯的降解特性。结果表明，降解酶在40℃、pH7．5时对氯氰菊酯显示最大的降解活性，对氯氰菊酯的降解率为78％；它在30～50C或pH6．5～8．0稳定性良好。Lineweaver-Burg法做图分析表明，其酶蛋白最大降解速率Vmax为63.69nmol·mL^-1·min^-1，米氏常数（Km）为377．35nmol／mL。
By pure culture, ultrasonic fragmentation and centrifuge separation,degradation enzyme for cypermethrin were extracted from the isolated stain w10j15 which had been identified as Enterobacter cloacap. Degradation characteristics of cypermethrin by enzyme were determined. The crude enzyme had optimum pH 7. 5 and temperature 40℃ for degradation of cypermethrin, and 78% of total cypermethrin was degraded. And it had its steadiness under pH6. 5～8.0 or 30～50℃. From the figure of Lineweaver-Burk, It was found that the Vmax for the degradation of cypermethrin was 63.69 nmol · mL^-1 · min^-1 and the Michaelis-Mentn constant was 377.35 nmol/mL.
出处
《西北农业学报》
CAS
CSCD
北大核心
2006年第1期64-67,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
加拿大国际发展署(CIDA)资助项目(098/S47074-192)
襄樊学院基金项目(KJ04043)
关键词
氯氰菊酯
阴沟肠杆菌
酶促降解
米氏常数
Cypermethrin
Enterobacter cloacap
Enzymatic degradation
Miehaelis-Mentn constant
