摘要
目的:观察趋化因子胸腺表达趋化因子(CCL25)和神经趋化蛋白(CX3CLl)在正常大鼠肝脏组织和肝纤维化大鼠肝脏内的表达变化。方法:研究正常肝组、肝纤维化造模4周组和肝纤维化造模6周组,每组10例,以ELISA法测定大鼠肝脏组织中CCL25和CX3CL1的含量。结果:CCL25在正常肝组、肝纤维化造模4周组和肝纤维化造模6周组的含量分别为(5.1±1.4)ng/g、(11.5±3.3)ng/g、(15.2±3.5)ng/g,肝纤维化组的含量显著高于正常肝组。CX3CL1在正常肝组、肝纤维化造模4周组和肝纤维化造模6周组的含量分别为(3.1±1.3)ng/g、(9.9±2.5)ng/g、(10.4±2.7)ng/g,肝纤维化组的含量均显著高于正常肝组(P<0.01)。结论:趋化因子CCL25和CX3CLl在大鼠的正常肝组织中有表达,但表达的数量水平较低。肝脏发生纤维增生性损伤时,肝内CCL25和CX3CL1的含量都显著增加,随着病变由肝纤维化向肝硬化阶段发展,肝内CCL25和CX3CLl的含量呈现出不同变化,CCL25表现为进一步升高,CX3CL1表现为维持在高水平。
Objective :To investigate the intrabepatic level of chemokine CCL25 and CX3CL1 in normal and hepatic fibrosis rats.Method :Thirty rats were divided into three groups: normal liver group, 4 weeks liver fibrosis group and 6 weeks liver fibrosis model group(n = 10).The content of CCL25 and CX3CL1 in hepatic tissue was assayed by ELISA.Results :The levels of CCL25 in the normal liver group, hepatic fibrosis model group in 4 weeks and 6 weeks were(5.1 ± 1.4), ng/g, (11.5 ± 3.3) ng/g, (15.2 ± 3.5) ng/g, respectively, and the levels in the liver fibrosis groups were significantly higher than those in the normal liver group. The contents in CX3CL1 in the normal liver group, hepatic fibrosis model group in 4 weeks and 6 weeks were (3.1 ± 1.3) ng/g, (9.9 ± 2.5) ng/g, and (10.4 ± 2.7 ng/g), respectively and the contents in liver fibrosis groups were significantly higher than those in the normal liver group, whose difference was statistically significant. Conclusion: The chemokine CCL25 and CX3CLl in rat liver tissue were in the normal expression, but the number was at the low level of expression. While fibrosis of liver injury occurred, the liver content of CCL25 and CX3CL1 significantly increased, and with the disease development from hepatic fibrosis stage into cirrhosis one, liver CCL25 and CX3CLl showed different changes in the content, CCL25 expression was further increased, CX3CL1 expression was maintained at a high level.
出处
《上海医药》
CAS
2012年第15期26-28,共3页
Shanghai Medical & Pharmaceutical Journal
