摘要
随着纳米氧化锌的广泛使用,其生物安全性备受关注。以人正常肺上皮细胞BEAS-2B为研究模型,采用WST-1检测技术,研究纳米氧化锌对该细胞的体外生物学毒性;同时,通过检测胞内ROS和胞外LDH释放水平来探讨其作用机制。研究发现,12.5~100μg/m L浓度范围内,纳米氧化锌对BEAS-2B细胞存在剂量依赖性的毒性作用,并导致BEAS-2B胞内ROS水平和胞外LDH释放显著性增加。这证实了纳米氧化锌的毒性作用与氧化应激效应有密切联系,该研究成果为深入研究和评价纳米氧化锌的生物安全性提供了重要的实验依据。
With the wide use of Nano Zinc Oxide, its biological safety is concerned. In normal human lung epithelial cell line BEAS-2B as the research model, the biologicaltoxicityin vitro of nano Zinc Oxide cells on it was studied by using WST-1 detection technology,and the mechanism of ROS by detecting intracellular and extracellular LDH release level was explored. This study found that in the range of 12.5 to 100 g/m L, Nano Zinc Oxide had a dose-dependent toxicity effect on BEAS-2B cells, and resulted in a significant increase in ROS levels and extracellular LDH release in BEAS-2B cells.It was confirmed that the toxicity of Nano Zinc Oxide had a close relationship with the oxidative stress effect,the research results can provide an important experimental basis for further research and evaluation of the biological safety of nano- Zinc Oxide.
出处
《生物化工》
2016年第6期44-46,共3页
Biological Chemical Engineering
关键词
纳米氧化锌
细胞毒性
氧化应激
Nano Zinc Oxide
Cytotoxicity
Oxidative stress
