摘要
目的探讨新型骨植入型抗生素释放系统硫酸钙/庆大霉素的抗生素释放特点。方法对研制的庆大霉素3%,5%含量的硫酸钙/庆大霉素抗生素释放系统进行抗生素洗提,应用高效液相色谱-蒸发光散射检测法(HPLC-ELSD)对不同时间点洗提液中庆大霉素含量进行测定,并与PMMA抗生素释放系统进行比较,观察抗生素释放特点。结果硫酸钙/庆大霉素抗生素释放系统与PMMA抗生素释放系释放时间分别为4周和2周,初期抗生素释放浓度高,相同时间点5%硫酸钙/庆大霉素释放系统抗生素释放量高于3%组,硫酸钙/庆大霉素组高于PMMA/庆大霉素组。结论硫酸钙/庆大霉素抗生素释放系统可降解,抗生素释放时间长,浓度高,优于PMMA抗生素释放系统。
Objective To discuss the elution characteristics of gentamycin from bone-implanted local antibiotic delivery system, Calcium Sulfate/Gentamycin. Methods To elute the gentamycin from the antibiotic delivery system, Calcium Sulfate/Gentamycin(Ca S/GM) and PMMA/ Gentamycin(PMMA/GM)delivery system, the concentration of gentamycin in the eluted fluid in different time was determined by HPLC. The elution characteristics of different delivery system in vitro were compared. Results The releasing time of Gentamycin frome Ca S/GM and PMMA/GM lasted for 4 weeks and2 weeks individually, the concentration of gentamycin in the eluted fluid during early periods was higher than that during later periods. In the same time gentamycin was released more from the 5% Ca S/GM than from 3% Ca S/GM. Comparing with PMMA/GM delivery system, gentamycin was also released more from Ca S/GM. Conclusion Ca S/GM antibiotic delivery system can be biodegraded, and the releasing time is longer and the releasing amount of gentamycin is higher from Ca S/GM than that from PMMA/GM individually, Ca S/GM antibiotic delivery system is superior to PMMA/GM system on antibiotic release and biodegradation.
出处
《生物骨科材料与临床研究》
CAS
2015年第1期15-18,后插3,共5页
Orthopaedic Biomechanics Materials and Clinical Study
基金
十一五全军医药卫生科研面上项目06MA261
关键词
局部抗生素释放系统
硫酸钙
庆大霉素
释放
生物降解
高效液相色谱法
Local antibiotic delivery system
Calcium sulfate
Gentamycin
Biodegradation
High performanceliquid chromatography(HPLC)
