摘要
研究了几种动物源性食品中氯霉素残留量的气相色谱测定方法;样品中氯霉素残留用乙酸乙酯提取,浓缩至干,溶于氯化钠溶液,用正己烷脱脂,水层再用乙酸乙酯提取,浓缩后用N,O-双(三甲基硅烷基)三氟乙酰胺(BSTFA)+1%三甲基氯硅烷(TMCS)衍生,用外标法定量;采用不同极性的色谱柱定性。当添加水平为0.3×10-9～10×10-9(w)时,回收率大于80%,相对标准偏差小于20%,线性相关系数r＝0.999。
A method for the determination of chloramphenicol residues in animal-origin food samples by gas chromatography(GC)with double electron capture detector is described.The sample pretreatment procedure inˉcludes extraction with ethyl acetate,evaporation of the extracts to dryness,dissolution of residues in aqueous sodium chloride solutions,extraction with n_hexane for the removal of fats,re_extraction of chloramphenicol into the ethyl acetate medium,and concentration of the ethyl acetate solution.The chloramphenicol in the concentratˉed ethyl acetate solution was finally derivatized with99%N,O_bis(trimethylsilyl)trifluoroacetamide(BSTFA)-1%trimethylchlorosilane(TMCS).Good repetitivity can be obtained with a RSD less than20%.The limit of detection is0.2μg/kg.At spiked levels of0.3×10 -9 ～10×10 -9 (w),the mean recovery exceeds80%.The linear correlation coefficient r equals to0.999.The method is cheap,rapid and sensitive,and can be applied to routine analysis.
出处
《分析测试学报》
CAS
CSCD
北大核心
2003年第5期105-107,共3页
Journal of Instrumental Analysis
