摘要
桑树内生真菌中筛选出产α-葡萄糖苷酶抑制剂的真菌,具有广阔的应用前景.文章从桑树根、茎、叶中分离得到10株内生真菌,利用体外酶活性测定方法筛选得两株具有α-葡萄糖苷酶抑制效果的菌株菌5和菌9.经菌落特征、显微镜观察和分子生物学鉴定,菌5和菌9为Aspergillus flavus(黄曲霉菌).
Screening ofα-glycosidase inhibitor from mulberry endophytic fungi has broad application prospects.Ten endophytic fungi strains were isolated from mulberry root,stem and leaf in this study.Two strains,i.e.,strain 5 and strain 9,containingα-glycosidase inhibition effect,were obtained by vitro enzyme activity determination method.The strain 5 and strain 9 were both identified as Aspergillus flavus through the colony characteristics observation,microscope observation and molecular biology identification.
出处
《浙江树人大学学报（自然科学版）》
2017年第1期24-27,共4页
Journal of Zhejiang Shuren University(Acta Scientiarum Naturalium)
基金
2016年度浙江省大学生科技创新项目(2016R421029)
关键词
Α-葡萄糖苷酶抑制剂
桑树
内生真菌
筛选
分子生物学鉴定
α-glycosidase inhibitor
Mulberry
endophytic fungi
screening
molecular biology identification
