摘要
以优良的库尔勒香梨为原料,使用YEPD培养基,从中分离获得100株酵母菌,按照菌落特征,形态学分析并结合ITS区基因序列同源性分析确定其种属,确定其为葡萄汁有孢汉逊酵母(Hanseniaspora uvarum)和奥默毕赤酵母(Pichia kudriavzevii),并对其进行耐酸、耐糖、耐乙醇和耐二氧化硫的分析。结果表明,菌株D4的最高耐糖含量为18%、菌株13为14%,菌株D4耐受pH值为3.0、菌株13耐受pH值为3.5,菌株D4、13最高耐乙醇体积分数均为9%,菌株D4的最高耐二氧化硫含量为0.20%、菌株13为0.15%。
Using excellent Korla pears as raw material, 100 strains of yeasts were isolated fxom Koala pears by YEPD medium. The strains were identi- fied according to the characteristics of colony and morphological analysis and the homology analysis of ITS gene sequence. Results showed that Hanseniaspora uvarum and Pichia kudriavzevii were confirmed and then their acid, sugar, ethanol and sulfur dioxide tolerance were tested. The re- suits showed that for strain D4, the highest sugar, pH, ethanol concentration and sulfur dioxide tolerances were 18%, 3.0, 9% and 0.20%, respectively. For strain 13, the tolerances mentioned above were 14%, 3.5, 9% and 0.15%, respectively.
出处
《中国酿造》
CAS
北大核心
2017年第6期67-71,共5页
China Brewing
基金
兵团科技攻关计划(2016AB009)
青年科学基金项目(31500092)
兵团科技攻关与成果转化计划项目(2016AD024)
高层次人才科研启动资金专项(RCZX201526)
