摘要
以蜂花粉为原料,用石油醚回流脱去脂类和色素,再用80%乙醇80℃回流2次提取蜂花粉黄酮;滤渣用80℃水提3次后,醇沉后,并用sevag法脱蛋白。计算多糖和黄酮的提取率和纯度,并对试验方法的重复性、精密度和稳定性进行考查。蜂花粉中黄酮的提取率为2.56%(RSD=1.722%,n=5),纯度为82.3%(RSD=2.517%,n=5),多糖提取率为3.11%(RSD=2.826%,n=5),纯度为91.6%(RSD=1.992%,n=5);精密度试验RSD_(黄酮)=1.760%,RSD_(多糖)=1.507%,稳定性试验中显色后的90 min内稳定性较好,RSD_(黄酮)=2.947%,RSD_(多糖)=2.632%。
This experiment by bee pollen as raw material,using petroleum ether reflux lipid and pigment,with two 80 ℃ 80 % ethanol reflux extraction bee flowers pink yellow ketone;with 80 ℃ water residue after three times,after alcohol sink,by sevag method to take off the protein. Calculation of polysaccharides and flavones extraction yield and purity,and the experimental method of repeatability,precision and stability test. Bee pollen medium yellow ketone extraction yield of 2.56 %(RSD = 1.722 %,n = 5),the purity of 82.3 %(RSD =2.517 %,n = 5),the polysaccharide extraction rate was 3.11 %(RSD = 2.826 %,n = 5),the purity of 91.6 %(RSD = 1.992 %,n = 5);Precision test RSD = 1.760 % of flavonoids,polysaccharide RSD = 1.507 %,stability test of color within 90 min after stability is better,flavonoids RSD = 2.947 %,polysaccharide RSD = 2.632 %.
出处
《食品研究与开发》
CAS
北大核心
2017年第10期59-62,共4页
Food Research and Development
关键词
蜂花粉
多糖
黄酮
联合提取
bee pollen
polysaccharide
flavonoids
combined extraction
