摘要
植物蛋白广泛应用于食品加工中,相关食品标准迫切需要建立蛋白质定性、定量的检测方法保证食品质量和安全。采用高效毛细管电泳法,对4种样品中的蛋白质进行检测,并利用指纹图谱软件对样品中所含蛋白种类进行比对,找出同类样品中共有特征峰,以及对不同植物蛋白互相掺假谱图进行比对,通过特征峰及保留时间对掺假物质进行鉴别,为植物蛋白掺假鉴别实验提供科学依据。
As plant proteins are widely used in food processing, analytical methods for the qualification and quantification of these proteins are urgently needed for food standards to ensure food quality and safety. In this experiment, protein contents were determined in four samples using capillary electrophoresis and protein identification was performed by comparison with standard fingerprints to find out common characteristic peaks. Moreover, mixed plant proteins were chromatographed and identified by comparison of characteristic peaks and retention times. This study may lay the foundation for the identification of plant protein adulteration.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第12期124-127,共4页
Food Science
基金
国家质检总局科技计划项目(2010QK109)
关键词
高效毛细管电泳
检测
植物蛋白
特征蛋白质
high performance capillary electrophoresis
detection
plant protein
characteristic protein
