摘要
采用富集培养法,从长期受氯氰菊酯农药污染的果园土壤中,分离筛选得到1株可降解氯氰菊酯的菌株N-02。经形态、生理生化特征及16S rDNA序列分析,初步鉴定为点状气单胞菌(Aeromonas punctata)。菌株N-02在35℃、pH 7.5、氯氰菊酯质量浓度为20 mg/L的LB培养基中培养96 h,对氯氰菊酯的降解率达到62.31%。该菌株N-02对拟除虫菊酯的降解谱较宽,培养96 h对20 mg/L高效氯氰菊酯、溴氰菊酯和氟氯氰菊酯降解率分别达到40.17%、33.26%和30.45%。
A bacterial strain named N-02 capable of degrading cypermethrin was isolated from long-term cypermethrin contaminated orchard soil by using enrichment culture. Based on the morphology, physio-biochemical characteristics, and 16S rDNA sequence analysis, strain N-02 was identified as Aeromonas punctata. The degradation rate of cypermethrin was 62.31% when the strain were cultured at 35 ℃ for 96 h in LB medium containing 20 mg/L cypermethrin at pH 7.5. A wide degradation spectrum of pyrethroid by strain N-02 was observed, and the degradation rates of β-cypermethrin, deltamethrin and fluoride cypermethrin after 96 h were 40.17%, 33.26% and 30.45%, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第11期160-163,共4页
Food Science
基金
西华大学重点科研基金项目(Z1310525)
四川省教育厅自然科学一般项目(14ZB0122)
关键词
氯氰菊酯
点状气单胞菌
生物降解
降解底物谱
cypermethrin
Aeromonas punctata
degradation characteristics
degradation spectrum
