摘要
从农药厂活性污泥中,分离到一株能以高效氯氰菊酯为唯一碳源生长的细菌CH7。经生理生化试验和16S rD-NA分析,将菌株CH7鉴定为铜绿假单胞菌(Pseudomonas aeruginosa)。采用Box-behnken设计试验、响应面法(response surfacemethodology)优化菌株CH7的降解条件。在最优条件下(29.4°C,pH7.0,接种量0.15g/L),菌株CH7在12d内对100mg/L高效氯氰菊酯的降解率为90%。
Strain CH7, isolated from activated sludge, could utilize beta-cypermethrin as the sole carbon resource for growth. Based on the results of physiological-biochemical properties and phylogenetic similarity of 16S rDNA gene sequences,strain CH7 was identified as Pseudomonas aeruginosa. Response surface methodology based on the Box-behnken design of experiment was used to optimize the degrading conditions of strain CH7. Under the optimum degrading conditions（29.4 C,pH7.0,biomass 0.15 g/L）, strain CH7 could degrade 90% beta-cypermethrin（ 100 mg/L）within 12 days.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第1期99-102,117,共5页
Biotechnology Bulletin
基金
国家高科技研究发展计划("863"计划)(2008AA10Z402
2006AA10Z402)
关键词
高效氯氰菊酯
铜绿假单胞菌
响应面法
降解条件优化
Beta-cypermethrin Pseudomonas aeruginosa Response surface methodology Degrading condition optimization
