摘要
以壳聚糖为载体、戊二醛为交联剂固定化β-半乳糖苷酶,通过单因素和正交实验探讨了固定化载体和固定化条件对酶固定化的影响。结果表明,固定化载体壳聚糖(脱乙酰度90%以上)的最适分子质量和体积分数分别为3×105和2%,制备的壳聚糖载体具有良好的成球性和机械强度。采用交联方式将β-半乳糖苷酶固定在壳聚糖微球上,在单因素试验的基础上,进行正交试验确定固定化条件为:交联剂戊二醛浓度和交联时间分别为10 g/L和1.0 h,酶浓度和固定化时间分别为1.5 mg/m L和12 h,最终制备的固定化酶的活力回收率达到70.5%。同时该固定化酶具有良好的储存稳定性和操作稳定性,具有一定的应用价值。
β-galactosidase was immobilized on chitosan cross-linked with glutaral-dehyde. The orthogonal experiment was adopted to determine immobilized carrier and the immobilization conditions. The results showed that the suitable molecular weight and concentration of immobilized carrier( degree of deacetylation of chitosan was more than 90%)were 3 × 105 and 2%( v / v). Chitosan microsphere carrier had good ballabililty and mechanical strength. β-galactosidase was immobilized on chitosan microspheres using cross-linking method. The immobilization conditions were as follows:glutaraldehyde concentration and crosslinking time were 10 g / L and 1 h,enzyme concentration and immobilization time were 1. 5 mg / m L and 12 h,the activity recovery of the immobilized enzyme reached 70. 5%. At the same time,the immobilized enzyme had good storage stability and operation stability,which had certain application value.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第5期105-109,共5页
Food and Fermentation Industries
基金
天津市科技计划项目(13ZCZDTG03400)
