摘要
建立HPLC测定淫羊藿苷壳聚糖微球中淫羊藿苷含量的方法。采用HPLC法,色谱柱：Marchal C18分析柱（250mm×4.6mm,5μm）,以乙腈-水（3070）为流动相,流速为0.8mL·min^-1,柱温为30℃,检测波长为270nm,进样量为20μL,测定3批淫羊藿苷壳聚糖微球中淫羊藿苷的含量。在此色谱条件下,淫羊藿苷可与其他成分较好的分离,峰型对称,淫羊藿苷标准品在2~125μg·mL^-1范围内与峰面积呈良好的线性关系（R2=0.999 7）,样品平均回收率为99.77%,RSD为0.57%,精密度,稳定性,重复性的RSD均﹤2%。该法操作简单、准确性高、重复性好,可用于检测淫羊藿苷壳聚糖微球中淫羊藿苷的含量。
The method was established for determining the content of Icariin in Icariin-loaded Chitosan Mi- crospheres by high performance liquid chromatography（HPLC）. Three batches of Icariin-loaded Chitosan Microspheres were determined for the content of Icariin by HPLC. The samples were separated on Marchal C18 （250 mm×4.6 mm,5μm） at the column temperature of 30 ℃ ,and with the mobile phase composed of acetonitrile water （30：70） at a flow rate of 0.8 mL ·min^-1. The detection wavelength was 270 nm and the sampling volume was 20/11. It was found that Icariin could be isolated from the other components and the type of peak was symmetrical. Icariin showed a good linear relationship with the peak area at the range of 2 ~125μg/ml （R2 =0.999 7）. The average recovery of Icariin was 99.77% and RSD was 0.57% ,the RSDS of precision, stability, repeatability were less than 2%. The method is simple, accurate and reproducible. It could be used for the determinationofIcariininIcariin-loaded Chitosan Microspheres.
出处
《南昌大学学报（理科版）》
CAS
北大核心
2015年第6期569-572,共4页
Journal of Nanchang University(Natural Science)
基金
南昌大学分析测试中心基金资助项目(2013026)
